4-METHYLUMBELLIFERONE INDUCES APOPTOSIS IN HUMAN ACUTE LEUKEMIA CELL LINES

PIBUEL M; LEVERMAN MARTIN; HAJOS S; LOMBARDO T; PAPADEMETRIO D; LOMPARDIA S; DIAZ M.; MIHALEZ C; ALVAREZ ELIDA

Mar del Plata

Congreso; Reunion Anual SAIC; 2016

SAIC- SAI- SAFE

4-methylumbelliferone (4MU) is a derivative of cumarine that has been approved in Europe and Asia to treat biliary spasms. During the last years, it has been proposed as a potential new drug for the treatment of solid tumors due to its capacity to inhibit hyaluronan synthesis, but yet little is known about its effect on hematological malignancies. Previous work of our lab showed hat 4MU induced senescence in human chronic myeloid leukemia cell lines. Considering this, we hypothesize that 4MU would be a potential new drug for the treatment of leukemia. Therefore, the aim of this work was to evaluate the effect of 4MU on acute myelomonocytic leukemia cell line (U937) and on acute T-lymphoblastic leukemia cell line (Jurkat). Cells were exposed to 0.1, 0.5, 1 and 2 mM 4MU and the following parameters were analyzed: cell proliferation by 3H-T uptake, cell death by flow cytometry using FDA/IP stain, apoptosis by fluorescence microscopy using acridine orange/ethidium bromide and DAPI stain and Bcl-2 modulation by western blot. The results showed that 4MU abrogated both cells growth in a dose-dependent manner (p