IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
CALCINEURIN-DEPENDENT INDUCTION OF MARKERS OF UTERINE RECEPTIVITY BY ANGIOTENSIN II IN RAT ENDOMETRIAL STROMAL CELLS. THE ROLE OF THE TROPHOBLAST RENIN-ANGIOTENSIN-SYSTEM
Autor/es:
SCOTTO FLORENCIA; GROTZ, ESTEFANIA; PARRADO CECILIA; SALAVERRY, LUCIANA; GENTILE, MARÍA TERESA; REY-ROLDÁN, ESTELA; CANELLADA, ANDREA
Lugar:
Mar del Plata
Reunión:
Congreso; Congreso; VI Latin American Symposium on Maternal-Fetal Interaction and Placenta and V Latin American Symposium on Reproductive Immunology Meeting 2015; 2015; 2015
Institución organizadora:
SLIMP and LASRI
Resumen:
The involvement of angiotensin II (Ang II) in maximal decidualization has been reported.We demonstrated that Ang II activates the Ca2+ / calcineurin (CN)/ NFAT pathway in rat endometrial stromal cells (ESCs). Ang II modulates gene expression, proliferation and metalloprotease activity in ESCs that could be involved in decidualization. Objective: To analyze in vitro a) whether Ang II induces decidual reaction and uterine receptivity in ESCs, b) the involvement of calcineurin, and c) whether Ang II secreted by trophoblasts could mediate those effects. Methods: ESCs from rat uterus were cultured with Ang II or estrogen plus medroxyprogesterone acetate (E2+MPA), for 24 h or 6 days. ESCs were pretreated with Cyclosporin A to assess the involvement of CN. mRNA expression of desmin (decidual prolactin related protein (dprp)), and insulin growth factor binding protein 1 (Igfbp1), markers of decidualization, was measured by RT-PCR. mRNA and protein expression of HB-EGF and IGF-1, markers of uterine receptivity, were measured by RT-PCR and western blot. The rat choriocarcinoma-derived cell line Rcho-1, which can be differentiated in vitro into invasive trophoblasts, was cultured in the presence or absence of E2 (10-8 M) or MPA (1 mM) for 48 h. ESCs were then cultured with the trophoblast conditioned medium (TrCM) for 48 h in the presence or absence of the Ang II receptor antagonist Losartan. Results: Ang II induced (p