IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Evaluation of the effects of skin ultraviolet light exposure on BCG vaccination effectiveness.
Autor/es:
CAMPO, VALERIA; FRIEDRICH, ADRIÁN; MANUELLI, PAULA; PAZ, MARIELA; LEONI JULIANA; GONZALEZ MAGLIO, DANIEL
Lugar:
Buenos Aires
Reunión:
Congreso; IV LASID. LXIII Argentinean Immunology Meeting. II French Argentinean Immunology Meeting.; 2015
Institución organizadora:
Sociedad Argentina de Inmunologia
Resumen:
It is well known that ultravioletradiation (UVr) leads to immunosuppression, inhibiting contact hypersensitivity(CHS) reactions. However, theway UVr modulates immune responses against microorganisms and vaccines has beenpoorly explored. Previously, we have found that a single high UV dose (shUVd -400 mJ/cm2) promotes skin inflammation while it decreases CHS, incontrast to repetitive low UV doses (rlUVD - 4 consecutive days, 20 mJ/cm2)which does not induce inflammation and increases CHS.Our aim was to evaluate the effectof both types of irradiation on BCG vaccination effectiveness in vivo.SKH:1 hairless mice were irradiatedand 24h later immunized with intradermal BCG (1x106 CFU).Non-irradiated mice and non-vaccinated mice were used as control. Four weeksafter vaccination, delayed-type hipersensitivity (DTH) reactions were evaluatedand mice were bled and sacrificed. Cell proliferation and cytokine productionwere determined in vitro stimulatingspleen cells with 40 µg/ml [J1] of BCGlysate. DTH reaction and specific IgG antibodies were slightly, but notsignificantly, increased in shUVd exposed animals compared with rlUVd ones (1.6vs 1.1 mm and 0.92 vs 0.67 units of OD492 nm). In vitro T cell proliferation had no differences between groups,but IL-4 production was increased in rlUVd exposed animals compared with shUVdones (19.2 vs 6.3 pg/ml; p<0.05).These results show that UVr is ableto modulate immune responses to vaccination, while the impact in the responsemay depend on the vaccine potency. Further studies should be done in order toelucidate effects on other types of vaccines.