EFFECTS OF METFORMIN ON OXIDATIVE DAMAGE AND INFLAMMATORY RESPONSE CAUSED BY ULTRAVIOLET B RADIATION (UVB) IN HUMAN KERATINOCYTES

SOUZA NETO F; ADRIÁN FRIEDRICH; PAZ ML; JEFA FER; LEONI J; GONZÁLEZ MAGLIO DH

CABA

Congreso; I meeting LASID FAIC SAI; 2015

LASID FAIC SAI

Ultraviolet radiation (UVr) induces an inflammatory and oxidative response in epidermal exposed cells, due to mitochondrial and cytosolic alterations. Both responses are related to skin cancer development. The mitochondrial electron transport chain (one of the major cellular generators of ROS) is inhibited by Metformin, an oral anti-hyperglycemic agent derived from Galega officinalis, which also has anticancer effects, which are explained trough the activation of AMPK. The aim of this work was to evaluate the effects of Metformin on UV-induced inflammation and mitochondrial alterations.HaCaT cell line pre-treated with 1 mM Metformin or media were exposed to 25 mJ/cm2 of UVr and incubated for 24 hs with or without 1 mM Metformin (a total of 4 groups). Non irradiated treated cells were used as control. Mitochondrial membrane depolarization, superoxide (O2?-) and cytokine production were evaluated by flow cytometry and ELISA, respectively.Metformin was more effective when solely administered before irradiation compared to the other treatments. It increased metabolically active cells (21.5% vs. 12.4%) and decreased O2?- producing cells (56% vs. 76%) as well as it reduced IL-6 production (2300 vs 3300 pg/ml) compared to non-treated irradiated cells. No changes in IL-10 production were detected. Metformin treatment after irradiation was less effective in reducing UV-induced damage. All differences were statistically significant (p