IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Effect of selected parameters in hibridoma cells immobilized in sol-gel silica matrices
Autor/es:
DESIMONE MF, DE MARZI MC, ALVAREZ GS, GANEM B, MALCHIODI EL, DIAZ LE
Lugar:
Mar del Plata, Buenos Aires, República Argentina.
Reunión:
Congreso; XLIII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2007
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
The sol-gel process is an inorganic polymerization taking place in mild conditions, allowing the association of mineral phases with organic or biological systems. Optimization of the technological parameters affecting the mechanical properties and permeability of sol-gel matrices is essential to develop matrices with improved properties for cell immobilization. In this work, the effect of different parameters such as the concentration of different sol-gel precursors and immobilized cell density, on the function of hybridoma cells producing anti-peptidoglycan receptor protein (PGRP) I-á.monoclonal antibody (mAb) has been investigated. Cell morphology was conserved as it was observed by optical and fluorescence microscopy. Cellular activity of immobilized hybridoma cells was determined by the tetrazolium assay (MTT assay) and a LIVE/DEAD cell vitality fluorescent assay. Viability was 89.5% in tetrakis (2-hydroxyethyl) ortosilitate-derived matrices and 60.5% in tetraethoxisilane-derived matrices. No differences were observed between cell densities employed in this work (5x105 – 3x106 cells/ml). MAb production from immobilized cells was detected by ELISA even after 10 days post immobilization. The design of such novel devices with significant added value is a key factor when foreseeing industrial developments of sol-gel materials in medicinal science.