IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Differential effect of IFN-I on autophagy modulation in tumor pancreatic cells.
Autor/es:
PAPADEMETRIO D; CAVALIERE V; COSTANTINO S; ALVAREZ E.
Lugar:
Mar del Plata
Reunión:
Congreso; Reunion Anual Conjunta SAIC SAI; 2014
Institución organizadora:
SAI
Resumen:
Infiltrating ductal adenocarcinoma of the pancreas (PDA) accounts for over 95% of all exocrine pancreatic malignancies, with a median survival for patients is 4-6 months. IFN-I exhibit a variety of biological effects which are different from those on viral replication, such as antitumor activity. IFN-I are widely expressed tumors. They have been used for the treatment of more than 14 types of cancer, including some hematological malignancies and certain solid tumors. In pancreatic tumors, autophagy process constitutes a resistant mechanism to several pro-apoptotic stimuli such as treatment with gemcitabine. The aim of this study was to investigate the effect of IFN-I on autophagy process and line. For that, we evaluate the modulation of autophagy levels by dying with CytoID Green and LC3 levels by western blot. We observed that IFN induced autophagy meanwhile IFN inhibited this process. Gemcitabine induced moderated apoptotic death of MIAPaCa-2 cells, assayed by TUNEL. When cells are pretreated with IFN, apoptosis induced by gemcitabine decreased in a 50% at all doses tested (p