IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Differential effect of IFN-I on autophagy modulation in tumor pancreatic cells.
Autor/es:
PAPADEMETRIO D; CAVALIERE V; COSTANTINO S; ALVAREZ E.
Lugar:
Mar del Plata
Reunión:
Congreso; Reunion Anual Conjunta SAIC SAI; 2014
Institución organizadora:
SAI
Resumen:
Infiltrating ductal adenocarcinoma of the pancreas (PDA) accounts for over 95% of all exocrine pancreatic malignancies, with a median survival for patients is 4-6 months. IFN-I exhibit a variety of biological effects which are different from those on viral replication, such as antitumor activity. IFN-I are widely expressed 􀁃􀁐􀁆􀀂􀁕􀁇􀁅􀁔􀁇􀁖􀁇􀁆􀀂􀁃􀁕􀀂􀁖􀁊􀁇􀀂􀅿􀁔􀁕􀁖􀀂􀁎􀁋􀁐􀁇􀀂􀁑􀁈􀀂􀁆􀁇􀁈􀁇􀁐􀁕􀁇􀀂􀁃􀁉􀁃􀁋􀁐􀁕􀁖􀀂􀁕􀁇􀁘􀁇􀁔􀁃􀁎􀀂􀁖􀁛􀁒􀁇􀁕􀀂􀁑􀁈􀀂 tumors. They have been used for the treatment of more than 14 types of cancer, including some hematological malignancies and certain solid tumors. In pancreatic tumors, autophagy process constitutes a resistant mechanism to several pro-apoptotic stimuli such as treatment with gemcitabine. The aim of this study was to investigate the effect of IFN-I on autophagy process and 􀁋􀁖􀁕􀀂 􀁋􀁐􀆀􀁗􀁇􀁐􀁅􀁇􀀂 􀁑􀁐􀀂 􀁉􀁇􀁏􀁅􀁋􀁖􀁃􀁄􀁋􀁐􀁇􀀂 􀁅􀁇􀁎􀁎􀀂 􀁔􀁇􀁕􀁒􀁑􀁐􀁕􀁇􀀂 􀁑􀁐􀀂 􀀯􀀫􀀣􀀲􀁃􀀥􀁃􀀏􀀔􀀂 􀁅􀁇􀁎􀁎􀀂 line. For that, we evaluate the modulation of autophagy levels by dying with CytoID Green and LC3 levels by western blot. We observed that IFN􀁄 induced autophagy meanwhile IFN􀁅 inhibited this process. Gemcitabine induced moderated apoptotic death of MIAPaCa-2 cells, assayed by TUNEL. When cells are pretreated with IFN􀁄, apoptosis induced by gemcitabine decreased in a 50% at all doses tested (p