The orogastric vaccination with an attenuatedstrain of Salmonella Typhi, triggers an immune response that promotes metastatic cells elimination in the liver.

VENDREL ALEJANDRINA; TESSONE A; MENAY F; COCOZZA F; GRAVISACO F; GOIN J; LAROTONDA G; CANELLADA, ANDREA; MONGINI C; WALDNER C

Mar del Plata

Congreso; LIX Reunión Científica de la Sociedad Argentina de Investigación Clínica (SAIC) y LXII Reunión Científica de la Sociedad Argentina de Inmunología (SAI); 2014

SAI/SAIC

Previously, we showed that the administration of CVD 915 via the orogastric route (o.g.) 24 h before tumor challenge can prevent implantation of LM3 cells in the liver, in a mouse model of liver metastasis. Twenty days after tumor challenge, treatment with CVD 915 significantly decreased the occurrence of liver metastases and total tumor volume. The aim of this work is to elucidate the immune mechanisms involved in the antitumor effect. For this purpose, 1x109UFC of CVD 915 was inoculated via o.g. in a group of BALB / c mice, while another group received PBS as a control. After 24 h, mice were injected with 100000 LM3 cells (mammary adenocarcinomas) into the spleen in a surgical frame and underwent splenectomy. After 24 h of the o.g. gavage with the bacteria, we find an innate immune response activation at the liver, characterized by a significant increase in IFN-γ production (p