IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Expression pattern of hyaluronan and its receptors, CD44 and RHAMM, in gestational trophoblastic disease (GTD).
Autor/es:
M. MASCARÓ, E. ZOTTA, M.I. BIANCONI, S. OTERO, N. LAGO, S. LOMPARDÍA, E. ALVAREZ, G. JANKILEVICH, S.E. HAJOS
Lugar:
Chicago
Reunión:
Congreso; ISSTD World Congress XVII; 2013
Institución organizadora:
International Society of Trophoblastic Disease
Resumen:
Expression pattern of hyaluronan and its receptors, CD44 and RHAMM, in gestational trophoblastic disease (GTD)   M. Mascaró, B.S.1, E. Zotta, M.D, Ph.D2, M.I. Bianconi, M.D.3, S. Otero, M.D.3, N. Lago, M.D.4, S. Lompardía, B.S.1, E. Alvarez, Ph.D1, G. Jankilevich, M.D.3, S.E. Hajos, Ph.D1.   1Tumor Immunology Laboratory, Department of Immunology-IDEHU, FFyB, University of Buenos Aires (UBA)-CONICET, Argentina. 2Pathophysiology Laboratory, Medical School, UBA, Argentina. 3Clinical Oncology Unit, Hospital de Agudos ?Carlos G Durand?, Buenos Aires, Argentina. 4Experimental and Applied Pathology Center, Medical School, UBA, Argentina.   STUDY OBJECTIVE   Hyaluronan (HA) and its receptors, CD44 and receptor for hyaluronan-mediated motility (RHAMM), are components of extracellular matrix playing an important role in tumor progression involving proliferation, multidrug resistance, migration, invasion and metastasis. The aim of this work was to study the expression and distribution of these molecules in gestational trophoblastic disease (GTD).   MATERIALS AND METHODS   Paraffin sections of 9 complete hydatiform mole (CHM), 2 choriocarcinomas (CC), 2 placental site trophoblastic tumors (PSTT) and 4 normal first-trimester placentas (NP) were studied by immunohistochemistry for the expression of HA, CD44 and RHAMM. Hydatiform moles were classified upon p57kip2 protein expression.   RESULTS   In CHM, RHAMM immunoreactivity was found in the apical membrane of villi, the cytoplasm of villous cytotrophoblast and the membrane of extravillous trophoblast. Both latter signals were stronger than the ones detected from NP. Instead, HA immunoreactivity was found in villous stroma both in CHM and NP although in a different pattern. HA immunoreactivity was also found in the apical and basal membrane of villi only in CHM. CD44 failed to be expressed either in NP or in CHM. Interestingly, HA and CD44 expression were related to tumor stroma in trophoblastic tumors while RHAMM staining was found in the tumor cell membrane and in the nucleus in CC and in PSTT, respectively.   CONCLUSION   Our results suggest that HA, CD44 and RHAMM may be involved in the pathophysiology of GTD. Although a higher number of samples (including partial hydatiform mole) need to be evaluated, our findings suggest a potential role of HA to distinguish CHM of NP. RHAMM was over-expressed in the membrane of invasive associated (extravillous) trophoblast population in CHM compared to NP. Differences of RHAMM localization in tumor trophoblast might partly explain different potential invasiveness of such entities. Blocking agents of HA-RHAMM interaction might be useful as adjuvant therapy to resistant cases of GTD.   Key words Hyaluronan, CD44, RHAMM, Gestational trophoblastic disease.