Variación en la calidad de la respuesta inmune humoral en mujeres con fallas endócrino-ginecológicas

REY ROLDÁN E; CORTELEZZI M; NOLTING M; ONETTO C; STRAMINSKY V; GENTILE TERESA

Buenos Aires

Congreso; V Congreso Argentino de Endocrinología Ginecológica y Reproductiva (SAEGRE). II Encuentro Latinoamericano de Endocrinología Ginecológica y Reproductiva; 2006

Sociedad Argentina de Endocrinología Ginecológica y Reproductiva (SAEGRE).

Hormonal modulation of asymmetrically glycosilated IgG in endocrine-gynecology failure women. Andrea Custidiano, M. Cortelezzi, M. Nolting, I.de San Sebastián, T. Gentile y E. Rey-Roldán. IDEHU-CONICET, FFyB, UBA y Servicio de Endocrinología Ginecológica, Hospital de Clínicas J. San Martín. Asymmetrically glycosilated IgG (Asym IgG) molecules posses an oligosaccharide moiety which interferes with the antigen combination, so, these antibodies cannot trigger the effector mechanisms of the immune response. Previous works demonstrated that during pregnancy there is an increase in serum Asym IgG. Furthermore, estrogen, progesterone and glucocorticoids modulate asymmetric antibodies synthesis in murine hybridoma. Our aim was to evaluate the asymmetric/simmetric IgG proportion in endocrine-gynecology failure patients. We used sera from women with clinical symptoms and laboratory diagnose of hyperprolactinemia, hypotiroidism and polycystic ovarian syndrome (PCO) as well as sera from normal women as controls (ages: 20-40 years old). The percentage of Asym IgG was determined by ELISA test before and after Concanavalin A-Sepharose affinity chromatography. The results demonstrated: 1) When prolactin serum levels (PRL) were slightly elevated a significant increased in asymmetric/symmetric proportion was observed. However, the relation was not altered by higher prolactin levels (% Asym IgG: control: 27.1±1.8, n=7; PRL:24-50 ng/ml: 42.6±5.4, n=4, p<0.05;  PRL >50 ng/ml: 31.0±5.0, n= 5). 2) In hypotiroid patients an increase in Asym IgG percentage was detected (49.0±5.8 %, n=3, p<0.01). 3) In PCO women also a higher proportion of asymmetric antibodies was found (39.4±3.2 %, n=6, p<0.05). These data suggest that certain endocrine pathologies, for example, hyperprolactinemia, hypotiroidism and polycystic ovarian syndrome could modulate the percentage of asymmetric glycosilated IgG synthesis and, as a consequence, alter the immune response quality. Immune-endocrine interactions could play a role in the asymmetric antibody response.