Angiotensina II induce marcadores de decidualización y receptividad uterina en cultivos primarios de células estromales aisladas de útero de rata.

ABRAHAM M, PARRADO A, SACCODOSSI N, REY E, GENTILE T, CANELLADA A

Jornada; XV Jornadas Anuales de la Sociedad Argentina de Biología (SAB); 2013

The requirement for Ca2+ mobilization, increased prostaglandin production, and the involvement of angiotensin II (Ang II) for maximal decidualization in rats, was reported. We demonstrated previously that Ang II activated Ca2+ / calcineurin / NFAT pathway and COX-2 expressión in rat endometrial stromal cells (ESC). Ang II also modulated ESC proliferation and metalloprotease activity, that could be involved in the effect of Ang II in the decidualization of ESC. To determine whether Ang induced decidual reaction and uterine receptivity in vitro, we cultured ESC isolated from rat uterus with Ang II (or estrogen plus progesterone as positive control) during different times, and then measured desmine mRNA expression (as a marker of decidualization) and the mRNA and protein expression of HB-EGF and IGF-1 (as markers of uterine receptivity), by RT-PCR and western blot. To assess whether calcineurin activity was involved in the effect of Ang II, we pretreated ESC with CsA. ESC cultured with Ang II showed increased desmine expression and morphology compatible with decidualized cells. Ang II induced HB-EGF and IGF-1 expression. CsA inhibited the increase in desmine as well as HB-EGF and IGF-1 expression in ESC cultured with either Ang II or the estrogen plus progesterone combination. Induction of markers of decidualization and uterine receptivity by Ang II in vitro, appears to require calcineurin activity.