- Extracts of an argentinian plant used in antirheumatic folk medicine diminished osteoclast diferentiation induced by RANKL- stimulation of murine macrophages

ABRAHAM F; PARRADO C; SACCODOSSI N; RUGNA A; REY ROLDÁN E; GENTILE T; CANELLADA A

Córdoba

Congreso; LXI Reunión Anual de la Sociedad Argentina de Inmunología (SAI).; 2013

Sociedad Argentina de Inmunología

Smilax campestris Griseb (SM), ordinary known as zarzaparrilla blanca, is the most widely distributed Smilax specie in the north of Argentine. Decoction of roots and rhizomes of this plant are used as diuretic, diaphoretic and antirheumatic folk medicine. A bone-protective role of several plant polyphenols, such as flavonoids which are present in SM, was found in menopause and rheumatoid arthritis. The aim of the study was to determine whether SM aqueous extracts inhibit RANK ligand (RL)-elicited osteoclast differentiation of macrophages, a molecular mechanism induced during rheumatoid arthritis. RAW 264.7 murine macrophages were cultured during 5 days with RL (100 ng/ml) in the presence or absence of SM (1-10-100-1000 ng/ml). We measured: multinucleated, tartrate resistant acid phosphatase (TRAP) positive (osteoclast-like) cells, by cytochemistry; cathepsin K mRNA expression, by RT-PCR; matrix metalloproteinases (MMP) activity, by gel-zymography; and cell proliferation, by BrdU incorporation; in order to asses osteoclast differentiation of RAW cells. Treatment of RAW cells with SM diminished RL-induced osteoclast-like cells (OLCs), cathepsin K mRNA expression and MMP activity, in a dose dependent manner. Significative inhibition