IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Expression pattern of hyaluronan and its receptors, CD44 and RHAMM, in gestational trophoblastic disease
Autor/es:
MASCARÓ, MARILINA; ZOTTA, ELSA; BIANCONI, MARÍA INÉS; OTERO, SILVINA; LAGO, NÉSTOR; LOMPARDÍA, SILVINA; ALVAREZ, ELIDA; JANKILEVICH, GUSTAVO; HAJOS, SILVIA
Lugar:
Chicago, IL
Reunión:
Congreso; ISSTD World Congress XVII; 2013
Institución organizadora:
ISSTD
Resumen:
STUDY
OBJECTIVE
Hyaluronan (HA) and its receptors, CD44 and receptor
for hyaluronan-mediated motility (RHAMM), are components of extracellular
matrix playing an important role in tumor progression involving proliferation,
multidrug resistance, migration, invasion and metastasis. The aim of this work
was to study the expression and distribution of these molecules in gestational
trophoblastic disease (GTD).
MATERIALS
AND METHODS
Paraffin sections of 9 complete hydatiform mole (CHM),
2 choriocarcinomas (CC), 2 placental site trophoblastic tumors (PSTT) and 4
normal first-trimester placentas (NP) were studied by immunohistochemistry for
the expression of HA, CD44 and RHAMM. Hydatiform moles were classified upon
p57kip2 protein expression.
RESULTS
In CHM, RHAMM immunoreactivity was found in the apical
membrane of villi, the cytoplasm of villous cytotrophoblast and the membrane of
extravillous trophoblast. Both latter signals were stronger than the ones
detected from NP. Instead, HA immunoreactivity was found in villous stroma both
in CHM and NP although in a different pattern. HA immunoreactivity was also
found in the apical and basal membrane of villi only in CHM. CD44 failed to be
expressed either in NP or in CHM. Interestingly, HA and CD44 expression were
related to tumor stroma in trophoblastic tumors while RHAMM staining was found
in the tumor cell membrane and in the nucleus in CC and in PSTT, respectively.
CONCLUSION
Our results suggest that HA, CD44 and RHAMM may be
involved in the pathophysiology of GTD.
Although a higher number of samples (including partial hydatiform mole)
need to be evaluated, our findings suggest a potential role of HA to
distinguish CHM of NP. RHAMM was over-expressed in the membrane of invasive
associated (extravillous) trophoblast population in CHM compared to NP.
Differences of RHAMM localization in tumor trophoblast might partly explain
different potential invasiveness of such entities. Blocking agents of HA-RHAMM
interaction need to be evaluated as a novel coadjuvant therapy of GTD.