IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Oral immunotherapy using soy proteins modulates the allergic response in a mouse model of cow´s milk allergy
Autor/es:
SMALDINI P; ORSINI DELGADO L; CURCIARELLO R,; CANDREVA ANGELA; FOSSATI CA; DOCENA GH,; PETRUCCELLI S,
Lugar:
Lima
Reunión:
Congreso; X Latin American Congress o Immunology.; 2012
Institución organizadora:
Asociación Latinoamericana de Inmunología
Resumen:
 Food allergy is caused by a breakdown in immunologic tolerance mechanisms. There is no approved therapy for food allergies. In this work we aimed to induce oral tolerance to cow`s milk proteins (CMP) using CMP and cross-reactive soy proteins (SP) in a food allergy mouse model. Balb/c mice were gavaged for a week with CMP (CMP Tol) or with SP (PS Tol) prior to sensitization by oral administration of CMP with choleric toxin. Finally, mice were orally challenged with CMP. Immune response was evaluated with in vitro (serum specific IgE, IgG1, IgG2a and IgA level; cytokine secretion and mRNA expression, and induction of Foxp3 T cells in the intestinal mucosa and splenocytes) and in vivo assays (clinical score and cutaneous tests). We found a lower medium clinical score in tolerized mice with CMP or PS after the oral challenge than in sensitized mice, with a negative cutaneous test in tolerized mice. Specific IgE [OD 1,57±0,2 vs 1,06±0.3 (SP Tol) or 0,52±0,1 (CMP Tol); p<0.05] and IgG1 [OD 2,16±0,3 vs 1,2±0.4 (SP Tol) or 0,11±0,02 (CMP Tol); p<0.01] were suppressed in tolerized mice. IL-5 and IL-13 secretion was reduced in tolerized mice at the protein and mRNA levels. We found inducible Tregs in mucosa. In conclusion, oral immunotherapy based on cross-reactivity between CMP and SP can be used to down-modulate the allergic specific immune response in CMP-sensitized mice.