IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
A BRUCELLA MEMBRANE PROTEIN IMPROVES THE INMUNOGENICITY OF THE CO-ADMINISTERED ANTIGEN BY INCREASING ITS HALF LIFE IN ENDOSOMAL COMPARTMENTS.
Autor/es:
MIRTA LORENA CORIA; M. VICTORIA DELPINO; ANDRÉS E. IBAÑEZ; LAURA ALEJANDRA BRUNO; GABRIELA SOFIA RISSO; PAULA BARRIONUEVO; GUILLERMO HERNAN GIAMBARTOLOMEI; JULIANA CASSATARO
Lugar:
Campos do Jordao
Reunión:
Congreso; XXXVII Congress of the Brazilian Society of Immunology; 2012
Institución organizadora:
Sociedad Brasilera de Inmunología
Resumen:
spp. (Bp) co-administered as adjuvant increases antigen (Ag) cross-presentation by dendritic cells (DCs) and induces IFN-g producing CD8+ and cytotoxic T cells. We also found that this protein has significant sequence homology with protease inhibitors from other bacteria, therefore we hypothesized that it would inhibit lysosomal proteases activities in endosomal compartments increasing the half life of the co-administered Ag thus increasing its immunogenicity. Methods and results: We analyzed the biological activity of Bp against cysteine and aspartyl proteases (cathepsins) that constitute a subset of endocytic proteases using a fluorometric assay kit (Enzcheck) which contains casein as substrate. We found that Bp inhibited partially the activity of cathepsin D, L, C, and B (p