Increased levels of hyaluronic acid in bronchoalveolar lavage of patients with interstitial lung disease

G. M. ERNST; CORDO RUSSO R. I.; LOMPARDÍA S. L.; AUTERI S.; GALINDEZ F.; ÁLVAREZ É.; GRYNBLAT P; HAJOS S. E.

Washington DC

Congreso; Presentado en FOCIS 2011; 2011

Sociedad Americana de Inmunología Clínica

PP-097-12 Hyaluronan levels in bronchoalveolar lavage of micewith induced allergic asthmaS. E. Hajos, G. M. Ernst, R. C. Russo, S. Lompardia, E. M. C. Alvarez.Faculty of Pharmacy and Biochemistry, University of Buenos Aires,Buenos Aires, ArgentinaHyaluronan (HA) is an important constituent of the extracellular matrix, whichaccumulates during inflammatory lung disease like asthma. Low molecularweight (LMW-HA) but not high molecular weight HA (HMW-HA) have shownto induce inflammatory cytokines secretion. CD44 is its principal receptor. Theaim of this work was to analyze the role of HA-CD44 interaction in asthmainduced inflammation. For this purpose, we established a model of asthma byintranasal inoculation of Dermatophagoides pteronyssinus (Dp) in BALB/cmice, which produced a significant increase of eosinophils in bronchoalveolarlavage (BAL). Histochemical analysis showed that lungs of mice sensitizedwith Dp presented lymphoid infiltration associated to bronchial tissue, while controlmice presented a normal structure. Flow cytometry analysis showed that thepercentage of CD44+ cells in BAL was significantly increased in Dp treatedmice compared to non-treated ones (47.4+3.6 vs. 14.0+7.1%, p,0.05). HAlevels in BAL were measured by ELISA finding higher HA concentration in Dptreated animals compared to controls (5.68+1.58 vs. 0.51+0.11 ug/ml,p,0.05) while Dp mice pre-treated with either Budesonide or Dexametasoneshowed decreased HA levels (0.42+0.07 and 0.39+0.12 vs. 5.68+1.58 ug/ml, p,0.05 respectively). Migratory capacity of cells from BAL was also evaluatedshowing that Dp sensitized mice presented higher migration towardsLMW-HA as compared to RPMI (migration index 2.2+0.2 vs. 1) but not towardsHMW-HA. When BAL cells were preincubated with anti-CD44 antibody,migration towards LMW-HA was inhibited (migration index 1.1+0.2). We concludethat HA-CD44 interaction is involved in inflammation induced by allergicasthma emphasizing its importance for therapy.