IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
The protein moiety of the Brucella Omp19 lipoprotein, co-administered as oral adjuvant, induces a Th1 immune response.
Autor/es:
A. E. IBAÑEZ; CORIA, LORENA; K. A. PASQUEVICH; BARRIONUEVO PAULA; DELPINO, MARÍA VICTORIA; G. H. GIAMBARTOLOMEI; CASSATARO, JULIANA
Lugar:
Buenos Aires
Reunión:
Congreso; Brucellosis 2011, International Research Conference; 2011
Institución organizadora:
Asociación Argentina de Microbiología (AAM)
Resumen:
Previous results demonstrate that the protein moiety of the Brucella Omp19 lipoprotein (U-Omp19) administered by the oral route has self-adjuvant properties. These prompt us to study its mucosal adjuvant capacity using as a model antigen ovalbumin (OVA). For this purpose mice were immunized orally with OVA, U-Omp19+OVA, or choleric toxin (CT)+OVA. By flow cytometry we determined that the percentage of CD4+ and CD8+ T cells expressing α4β7 integrin increased in those mesenteric lymph nodes from U-Omp19+OVA (CD4+ α4β7+: 7,7%; CD8+ α4β7+:12,16%) and CT+OVA (7,15%; 16,46%) in comparison with OVA (0,74%; 1,64%) immunized mice. We also observed a significant delayed type hypersensitivity (DTH) response to OVA injection in U-Omp19+OVA (Δ=0,11mm, P