IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Use of a food allergy mouse model to study the in vivo and in vitro cross-reactivity between cow's milk and soybean proteins
Autor/es:
SMALDINI P; CURCIARELLO R,; CANDREVA ANGELA; FOSSATI CA; PETRUCCELLI S,; DOCENA GH,
Reunión:
Congreso; First French-Argentine Immunology Congress. LVIII Reunión Anual de la Sociedad Argentina de Inmunología; 2010
Resumen:
Treatment of food allergy
consists in allergen avoidance and food replacement. Cow´s milk (CM) is
frequently substituted for soy (S)-containing formulae, however it is not
tolerated in some patients even if they were not previously exposed to soy
proteins. Our group has identified caseins and soy globulins as the
cross-reactive allergens. They were obtained as recombinant proteins and
cross-reactive epitopes were mapped.The aim of this work was to employ a
validated food allergy mouse model to study the in vivo and in vitro
cross-allergenicity between CM and S.BALB/c mice were intragastrically
sensitized with CM proteins (CMP) and cholera toxin (CT) as a Th2 mucosal
adyuvant. Control group consisted of mice exposed to CMP or CT. An oral
challenge was performed with CMP or S, and 15-30 minutes afterwards clinical
signs were recorded (clinical score). Cutaneous tests were performed, specific
antibody (IgE, IgG1 and IgG2a) and histamine serum levels were quantified,
antigen-specific proliferation of splenocytes was analyzed and cytokine
production was measured. Sensitized animals showed higher clinical scores than
control mice, and both CMP and S induced symptoms. Specific IgE and IgG1
antibodies were increased in sensitized mice, while IgG2a was suppressed.
Histamine was increased and splenocytes were stimulated with CMP and S
extracts. IL-5 (CMP: 132 ± 64 pg/ml vs. nd, S: 29± 11 vs 7±3 pg/ml, sensitized
vs control group resp.) was also increased, while gIFN was basal (CMP: 80±16
vs. 26±13 pg/ml, S: 126±52 vs. 390±170 pg/ml sensitized vs control group
resp.). Cutaneous tests were positive for CMP and soy. In conclusion,
intragastric sensitization with CMP induced a specific Th2-bias activation of
the gut mucosa immune system that also reacted with soy proteins.
Cross-allergenicity was also evidenced by elicitation of clinical signs and
cutaneous tests. These results may constitute the experimental basis to develop
a mucosal vaccine