IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Quantification of IgM, total IgG, IgG1 and HCAbs in colostrum of llamas (Lama glama) by ELISA
Autor/es:
SACCODOSSI, NATALIA; VEIGA, FERNANDA; MEINCKE, FEDERICO; CHIAPPE, MARÍA, A. ; LEONI, JULIANA ; DE SIMONE, EMILIO
Lugar:
Buenos Aires
Reunión:
Congreso; 1st. French-Argentine Congress. LVIII Reunión Anual de la SAI; 2010
Institución organizadora:
Sociedad Argentina de Inmunología (SAI)
Resumen:
Quantification of IgM, total IgG, IgG1 and HCAbs  in colostrum of llamas (Lama glama) by ELISA  Saccodossi, Natalia; Veiga, Fernanda; Meincke, Federico; Chiappe M. Angelina; Leoni, Juliana; De Simone, Emilio  Camelids possess a particular kind of antibodies devoid of light chains (known as HCAbs). Three IgG isotypes were identified (IgG1, IgG2 and IgG3); IgG2 and IgG3 are HCAbs whereas the IgG1maintains the conventional structure. Due to the type of placentation in llamas, newborns required the ingestion and absorption of immunoglobulins (Igs) through colostrum to acquire passive immunity. Due the lack of specific reagents IgM and IgG isotypes had not yet been measured in colostrum. Objective: Determine the quantity of IgM, total IgG, IgG1 and HCAbs (IgG2 and IgG3) in colostrum of llamas. This represents an important tool for the study of the passive transference in llamas and contribute to the study of the biological functions of HCAbs. For this assay we obtained 12 samples of colostrum between the first day post partum.  Previously, in our group we developed a specific polyclonal antiserum against IgM, total IgG and IgG subisotypes. We used anti-IgM (H), anti-total IgG (H) and anti-IgG1(CH1) plyclonal antisera in order to design 3 types of ELISAs sandwich (to determine IgM, total IgG and IgG1). HCAbs’s quantity was calculated by difference between total IgG and IgG1 (HCAbs = total IgG – IgG1). The mean and the standard deviation (SD) of the results obtained are detailed below: IgM =18.02 mg/ml (SD=10.50), total IgG=42.09mg/ml (SD=30.18), IgG1=24.04mg/ml (SD=15.37) y HCAbs (IgGtotal-IgG1) = 18.05 mg/ml (SD=16.73). Concentrations of total IgG are similar to the ones described for species with similar placentation. Besides, if we considered IgG1/HCAbs ratio, it presents an important variation between individuals. However the concentration of IgG1 is higher than HCAbs in 8/12 of the samples. Finally the IgM showed far higher concentrations than the rest of mammals with similar types of placentation. At present we are developing an anti-IgA serum which enables us to quantify this isotype in colostrum.