IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Homeostatic effect of Lipoteichoic acid from L. rhamnosus GG after immunosuppressive UV irradiation.
Autor/es:
WEILL, FEDERICO ; CELA, ELIANA; PAZ, MARIELA; PERDIGÓN, GABRIELA; GONZÁLEZ MAGLIO, DANIEL ; LEONI, JULIANA
Lugar:
Buenos Aires
Reunión:
Congreso; 1st. French-Argentine Congress. LVIII Reunión Anual de la SAI; 2010
Institución organizadora:
Sociedad Argentina de Inmunología (SAI)
Resumen:
Homeostatic effect of Lipoteichoic acid from L. rhamnosus GG after immunosuppressive UV irradiation. Weill, Federico S; Cela, Eliana M; Paz, Mariela L; 1Perdigón, Gabriela; González Maglio Daniel H; Leoni, Juliana M. Affiliation: Cátedra de Inmunología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires; 1Centro de Referencia para Lactobacilos (CERELA-CONICET)   Immunosuppressive effect of UV radiation (UVR) has been largely described. Following skin UV exposure, immune response is biased towards a Th2/Treg profile. In contrast, probiotics have been largely described as beneficial for the host’s immune system. Lactobacilli are the best described probiotics and lipoteichoic acid (LTA) is a major cell wall antigen in this bacteria. Recently, skin homeostasis recovery of UV induced immunosuppressive effect with oral probiotics has been shown, reinforcing the idea of the existence of a gut-skin immune axis. The aim of our study was to evaluate the effect of the oral administration of LTA from L. rhamnosus GG (LrGG) on acute UV irradiated mice focusing on immunological parameters of immunosuppression. SKH:1 hairless mice were divided into 3 groups of 5. One group received 100µl of LTA (1mg/ml) and other group received 100µl/day of PBS for 10 days and the other group was untreated. Treated groups were irradiated with 400mJ/cm2 of UVB once and sacrificed 24hs later. ConA induced T cell proliferation was evaluated on inguinal lymph nodes (ILN) and spleen cells. IL-10, IL-4 and IFN-g were measured in the culture supernatants. ILN cells were stained with anti-CD3, CD4 and CD8 fluorescent Abs and epidermal cells with anti-CD3 and gd Abs for FACS analysis. While no difference was found in the proliferative response in either organ, IL-10 production (increased after UV radiation) in ILN was lower in LTA group than in PBS group (p<0,05). Additionally, ILN CD3CD4+ cell percentage was higher in LTA group than in PBS group (51,04±3,30 vs 45,77±2,30, p<0,05) and epidermal CD3gd+ was lower in LTA group than in PBS group (1,99±0,69 vs 3,02±0,96, p<0,05). This study shows that LrGG LTA administration reverts, at least partially, some of the immunosuppressive effects of UVR. Probably, this phenomenon is mediated by the effect LTA has on the GALT, which in turn results in a homeostatic systemic effect observed in the skin draining lymph nodes.