Brucella suis ΔmapB OUTER MEMBRANE VESICLES POTENTIAL AS VACCINE CANDIDATES AGAINST BRUCELOSIS.

BIALER, MAGALÍ G.; MUÑOZ GONZÁLEZ, FLORENCIA; BALDI, PABLO C.; ALONSO PAIVA, IVÁN M.; FERRERO, MARIANA C.

Congreso; Reunión anual de sociedades de biociencias; 2021

Sociedad Argentina de Inmunología

Outer membrane vesicles (OMVs) have been used in development of vaccines against Gram negative bacteria. Here we evaluated B. suis M1330 (wt) and B. suis ∆mapB OMVs immunogenicity and protective capacity against both systemic and mucosal B. suis challenge.Female Balb/c mice were immunized intramuscularly (i.m.) with OMVwt (20 μg), OMVΔmapB (20 μg) or saline at 0 and 30 days. One week after last immunization serum, bronchoalveolar lavage, feces and saliva samples were obtained to measure OMV-specific antibodies. One-week later mice were challenged with virulent B. suis through the intraperitoneal (i.p.) or intratracheal (i.t.) routes. CFU counts were determined in lungs and/or spleens 20 days after challenge. Antibodies capacity to neutralize Brucella infection was determined in lung epithelial cells culture (A549 cell line).Vaccination with both OMVs induced serum specific IgG (p