IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
artículos
Título:
Role of regulatory and angiogenic cytokines in invasion of trophoblastic cells.
Autor/es:
DUBINSKY V; POEHLMANN T,; SUMAN P; GENTILE T; MARKERT U; GUTIERREZ G
Revista:
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Oxford; Año: 2010 p. 193 - 199
ISSN:
1046-7408
Resumen:
PROBLEM: Trophoblast invasion is a temporally and locally restricted
process, which regulates implantation and oxygen arrival to the embryo
through the dialog with spiral artery endothelium. Trophoblast factors
with angiogenic potential are activated by hypoxia. Their capacities to
induce proliferation, migration, and invasion of trophoblastic cells
have been investigated. METHOD OF STUDY: The expression of interleukin
(IL)-6, CD126, CD130, vascular endothelial growth factor (VEGF), and
hypoxia inducible factor-1alpha (HIF-1alpha) has been silenced in JEG-3
choriocarcinoma cells by using siRNA. Silencing efficacy has been
assessed by ELISA, PCR or Western blotting. Proliferation has been
measured by flow cytometry, migration by a transwell assay, and
invasion by a Matrigel assay. RESULTS: Proliferation was significantly
reduced by silencing of CD126 or CD130, migration by silencing of IL-6,
VEGF, or HIF-1alpha, and invasion by silencing of IL-6 and HIF-1alpha.
CONCLUSION: The expression of IL-6, VEGF, and HIF-1alpha in
trophoblastic cells is involved in the control of trophoblast invasion
and migration.