IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
artículos
Título:
ROS production by endogenously generated protoporphyrin IX in murine leukemia cells
Autor/es:
B. DIEZ, R. CORDO RUSSO , M. J. TEIJO, S. HAJOS, A. BATTLE H. FUKUDA
Revista:
CELLULAR AND MOLECULAR BIOLOGY
Editorial:
C M B ASSOC
Referencias:
Lugar: USA; Año: 2009 vol. 55 p. 15 - 19
ISSN:
0145-5680
Resumen:
Endogenous production of Protoporphyrin IX (PpIX) is successfully exploited for photodynamic therapy (PDT)
on malignant cells, following 5-aminolevulinic acid (ALA) administration and light irradiation. This treatment kills cancer
cells by damaging organelles and impairing metabolic pathways via cellular reactive oxygen species (ROS) generation. We
studied the efficiency of PpIX synthetized from ALA on ROS generation, in the Vincristine resistant (LBR-V160),
Doxorubicin resistant (LBR-D160) and sensitive (LBR-) murine leukemia cell lines. Cells were incubated 4 hr with 1 mM
ALA and then irradiated during different times with fluorescent light. One hour later, production of ROS was analyzed by
flow cytometry using different fluorescent probes: Hydroethidine (HE) for superoxide anion, 2,7
Dichlorodihydrofluorescein diacetate (DCFH-DA) for hydrogen peroxide; mitochondrial damage was examined with 3,3
Dihexyloxacarbocyanine iodide (DiOC6). We found that superoxide anion production in the three cell lines increased with
irradiation time whereas no peroxide hydrogen was detected. Mitochondrial damage also increased in an irradiation time
dependent manner, being higher in the Vincristine resistant line. Previous studies have demonstrated that apoptotic cell death
increased with irradiation time, which is consistent with these results, indicating that ROS are critical in ALA-PDT
efficiency to kill malignant cells.