IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
artículos
Título:
Proinflammatory response of canine trophoblasts to Brucella canis infection
Autor/es:
FERNÁNDEZ, AG; FOSSATI, CA; FERRERO, MC; HIELPOS, MS; BALDI, PC
Revista:
PLOS ONE
Editorial:
PUBLIC LIBRARY SCIENCE
Referencias:
Lugar: San Francisco; Año: 2017 vol. 12 p. 186561 - 186561
ISSN:
1932-6203
Resumen:
Brucella canis infection is an important cause of late-termabortion in pregnant bitches. The pathophysiological mechanisms leading to B. canis?induced abortion are unknown,but heavily infected trophoblasts are consistently observed. As trophoblastsresponses to other pathogens contribute to placental inflammation leading to abortion, the aim of thepresent study was to characterize the cytokine response of canine trophoblaststo B. canis infection. To achievethis, trophoblastsisolated from term placenta of healthy female dogs were infected with B. canis, culture supernatants wereharvested for cytokine determinations, and intracellular B. canis location was evidenced by indirect immunofluorescence withan anti-B. canis antibody. Additionally,cytokine responses were assessed in non-infected trophoblasts stimulated withconditioned media (CM) from B. canis-infectedcanine monocytes and neutrophils. Finally, cytokine response and bacteriareplication were assessed in canine placental explants infected ex vivo. B. canis successfully infected and replicated in primary caninetrophoblasts, eliciting an increase in IL-8 and RANTES secretion. Moreover, the stimulation of trophoblasts withCM from B. canis-infected monocytesand neutrophils induced a significant increase in IL-8, IL-6 and RANTESsecretion. B. canis replication wasconfirmed in infected placental explants and the infection elicited anincreased secretion of TNF-α, IL-8, IL-6 and RANTES. This study shows that canine trophoblastsproduce proinflammatory cytokines in response to B. canis infection and/or to stimulation with factors produced byinfected monocytes and neutrophils. These cytokines may contribute to placentalinflammation leadingto abortion in B. canis-infectedpregnant bitches.