Expression of HLA-G and MICA mRNA in renal allograft

A RACCA, C VEAUTE,A BAILAT, L GAITE, M ARRIOLA, S HAJOS, I MALAN BOREL

TRANSPLANT IMMUNOLOGY

Elsevir

Lugar: USA ; Año: 2009 vol. 21 p. 10 - 12

0966-3274

Abstract: HLA-G is a nonclassical MHC class I antigen that displays tolerogenic functions; MICA is a stress-regulated molecule recognized by NKG2D cytotoxicity-activating receptor expressed by NK and T cells subsets. We evaluated HLA-G isoforms and MICA mRNA levels in peripheral blood mononuclear cells (PBMCs) and in biopsies from kidney allograft recipients with acute rejection (AR), chronic rejection (CR), and stable graft evolution (SE). HLA-G1 was the only transcript resulted from amplification, both in PBMCs as in biopsy samples. HLA-G1 mRNA levels in PBMCs from 9/10 patients with CR, 7/9 with AR and 8/10 healthy volunteers were below the median value of SE patients. The analysis of biopsies revealed that patients with AR (n=6), who overcame rejection had a tendency towards higher HLA-G1 levels than those with nephrotoxic acute tubular necrosis (ATN) (n=3). Similar levels of MICA expression were observed in PBMCs from AR, CR, SE and C groups; MICA expression levels were similar also in biopsy specimens from AR and nephrotoxic ATN patients. No correlation was found between MICA expression and the graft state. These results suggest that HLA-G1 isoforms, but not MICA mRNA levels, may provide a marker for measuring the state of kidney allograft and perhaps its evolution. nephrotoxic ATN patients. No correlation was found between MICA expression and the graft state. These results suggest that HLA-G1 isoforms, but not MICA mRNA levels, may provide a marker for measuring the state of kidney allograft and perhaps its evolution. nephrotoxic ATN patients. No correlation was found between MICA expression and the graft state. These results suggest that HLA-G1 isoforms, but not MICA mRNA levels, may provide a marker for measuring the state of kidney allograft and perhaps its evolution. nephrotoxic ATN patients. No correlation was found between MICA expression and the graft state. These results suggest that HLA-G1 isoforms, but not MICA mRNA levels, may provide a marker for measuring the state of kidney allograft and perhaps its evolution.