ESTROGEN AND PROGESTERONE REGULATE THE IL-6 SIGNAL TRANSDUCTION PATHWAY

CANELLADA ANDREA; ALVAREZ I; BEROD L; GENTILE T

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY

Elsevier

Año: 2008 vol. 111 p. 255 - 261

0960-0760

Estrogen (E2) and progesterone (Pg) modulate the synthesis of blocking, asymmetric antibodies in a dose-dependent manner. Since IL-6 and a progesteroneinduced blocking factor (PIBF) were shown to stimulate the asymmetric antibody synthesis by an hybridoma B cells, in this work we analyzed whether E2 and Pg were able to modulate IL-6 signal transduction pathways and also, whether hybridoma B cells synthesized PIBF in response to Pg. It was found that while the IL-6 treatment induced an increase in the expresion of of gp130 and JAK-1 by the hybridoma, 10-6M E2 and Pg diminished IL-6-induced gp130 expression and phosphorylation whereas JAK-1 was not significantly affected. In adidition, 10-6M E2 and Pg diminished IL-6-induced STAT3 phosphorylation and traslocation to the nucleus of the hybridoma cells. Maximal PIBF expression was observed when the hybridoma was cultured with 10-10 M Pg, compared to the control (p<0.05). The results suggest that the effects of E2 and Pg on asymmetric antibody synthesis would be accomplished by a modulation of the IL-6R signal transduction pathways and PIBF induction.