IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
artículos
Título:
Effects of Enterococcus faecalis CECT7121 on the specific immune response after DTPw vaccination
Autor/es:
CASTRO MS; MOLINA MA; SPARO MD; MANGHI MA
Revista:
International Journal of Probiotics and Prebiotics
Editorial:
New Century Health Publishers, LLC
Referencias:
Año: 2008 vol. 3 p. 25 - 30
ISSN:
1555-1431
Resumen:
Enterococcus faecalis CECT7121 is a non-pathogenic strain that, after the intragastric (ig) administration, it colonises the intestinal tract of BALB/c mice and modulates the innate systemic immune response by inducing the synthesis of homeostatic cytokines (IL-12 and IL-10). In this work we analysed the adjuvant properties of E. faecalis CECT7121, as well as its influence on the Th1/Th2 polarisation during the specific immune response generated by the immunisation with the vaccine of paediatric use DTPw (diphtheria, tetanus and whole-cell Bordetella pertussis). The study was carried out comparatively in naïve BALB/c mice and mice pretreated ig with E. faecalis CECT7121 before the sc immunisation with DTPw. The following parameters were assessed: the levels of antitetanus and antidiphtheria antibodies, the proliferation of splenocytes and the levels of IFNg, IL-5, IL-6 and IL-12 secreted in culture supernatants after the specific stimulation with tetanus and diphtheria toxoids. Those animals pretreated with E. faecalis CECT7121 and immunized with DTPw displayed higher levels of secreted IFNg, IL-6 and specific proliferation than untretated animals (p<0.05). The levels of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained unaffected. Our results demonstrate that the ig administration of E. faecalis CECT7121 has an adjuvant effect and behaves as a pro-Th1 modulator inducing the synthesis of higher levels of IFNg in the antitetanus and antidiphtheria responses at the systemic level.