IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
artículos
Título:
Effects of Enterococcus faecalis CECT7121 on the specific immune response after DTPw vaccination
Autor/es:
CASTRO, M; MOLINA, M; SPARO, M; MANGHI, M
Revista:
International Journal of Probiotics and Prebiotics
Editorial:
New Century Health Publishers, LLC
Referencias:
Año: 2007 vol. 3 p. 25 - 30
ISSN:
1555-1431
Resumen:
Enterococcus faecalis CECT7121 is a nonpathogenic
strain that, after the intragastric (ig)
administration, it colonises the intestinal tract of BALB/c
mice and modulates the innate systemic immune response by
inducing the synthesis of homeostatic cytokines (IL-12 and
IL-10). In this work we analyzed the adjuvant properties of
E. faecalis CECT7121, as well as its influence on the Th1/
Th2 polarization during the specific immune response
generated by the immunization with the vaccine of pediatric
use DTPw (diphtheria, tetanus and whole-cell Bordetella
pertussis). The study was carried out comparatively in naïve
BALB/c mice and mice pretreated ig with E. faecalis
CECT7121 before the sc immunization with DTPw. The
following parameters were assessed: the levels of antitetanus
and antidiphtheria antibodies, the proliferation of splenocytes
and the levels of IFN γ, IL-5, IL-6 and IL-12 secreted in
culture supernatants after the specific stimulation with
tetanus and diphtheria toxoids. Those animals pretreated
with E. faecalis CECT7121 and immunized with DTPw
displayed higher levels of secreted IFN γ, IL-6 and specific
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
culture supernatants after the specific stimulation with
tetanus and diphtheria toxoids. Those animals pretreated
with E. faecalis CECT7121 and immunized with DTPw
displayed higher levels of secreted IFN γ, IL-6 and specific
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
culture supernatants after the specific stimulation with
tetanus and diphtheria toxoids. Those animals pretreated
with E. faecalis CECT7121 and immunized with DTPw
displayed higher levels of secreted IFN γ, IL-6 and specific
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
culture supernatants after the specific stimulation with
tetanus and diphtheria toxoids. Those animals pretreated
with E. faecalis CECT7121 and immunized with DTPw
displayed higher levels of secreted IFN γ, IL-6 and specific
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
culture supernatants after the specific stimulation with
tetanus and diphtheria toxoids. Those animals pretreated
with E. faecalis CECT7121 and immunized with DTPw
displayed higher levels of secreted IFN γ, IL-6 and specific
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
γ, IL-5, IL-6 and IL-12 secreted in
culture supernatants after the specific stimulation with
tetanus and diphtheria toxoids. Those animals pretreated
with E. faecalis CECT7121 and immunized with DTPw
displayed higher levels of secreted IFN γ, IL-6 and specific
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
γ, IL-6 and specific
proliferation than untretated animals (p<0.05). The levels
of IL-5 and IL-12 as well as the IgG1/IgG2a ratios remained
unaffected. Our results demonstrate that the ig
administration of E. faecalis CECT7121 has an adjuvant
effect and behaves as a pro-Th1 modulator inducing the
synthesis of higher levels of IFN γ in the antitetanus and
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
antidiphtheria responses at the systemic level.
γ in the antitetanus and
antidiphtheria responses at the systemic level.