Apoptosis induction and activation of signaling pathways in lymphoid malignancies

S.E. HAJOS, M. GARCÍA, V. CAVALIERE, R. C. RUSSO, L. ALANIZ, D. PAPADEMETRIO, G. BLANCO, E. ALVAREZ

MEDIMOND International Proceedings

ICI 2007 MEDIMOND

Lugar: Italia; Año: 2007

Murine multidrug resistant and human leukemic cell lines were used to investigate: a) modulation of NF-kB signaling pathway by BAY 11-7082, CAPE and MG-132 and their effect on apoptosis induction, b) the ability of HA and oHA to induce apoptosis on MDR cell lines by down regulation of PI3K/Akt pathway. We showed constitutive NF-kB activity in murine resistant cell lines and dependence of this pathway in the human cell line, suggesting that NF-kB signaling pathway should play a key role in proliferation and survival. Specific inhibitors down regulated NF-kB expression in a dose-dependent manner leading to apoptosis induction. oHA were also able to induce apoptosis in murine resistant cell lines through decreasing Akt phosphorylation as well as survivin levels.