IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
artículos
Título:
CHARACTERIZATION OF IGM, TOTAL IGG, IGG1 AND HCABS IN COLOSTRA OF LLAMAS (LAMA GLAMA) BY ELISA
Autor/es:
NICOLÁS CAGGIANO; NATALIA SACCODOSSI; TERESA GENTILE; MARÍA ANGELINA CHIAPPE BARBARÁ; JULIANA LEONI; EMILIO DE SIMONE
Revista:
Revista Complutense de Ciencias Veterinarias
Editorial:
Universidad Complutense de Madrid
Referencias:
Lugar: Madrid; Año: 2014 vol. 8 p. 29 - 40
ISSN:
1988-2688
Resumen:
ABSTRACT. Objectives: to determine the levels of IgM, total IgG, IgG1 and HCAbs (IgG2 and IgG3) in colostra of llamas and to evaluate the concentration of HCAbs in relation to total IgG and the conventional isotype IgG1. Methods: Fifteen pregnant llamas were used in this study, which were milked within the first 24 hours after delivery. Sandwich ELISAs were designed for the quantification of IgM, total IgG and IgG1. The HCAbs concentration was calculated by difference between total IgG and IgG1 (HCAbs = total IgG ? IgG1). Results: The levels of Igs were found to be: IgM= 17.02 mg/ml (SD= 9.85), total IgG= 42.54mg/ml (SD=27.79), IgG1=24.34mg/ml (SD=13.96) and HCAbs (IgGtotal-IgG1) = 18.19 mg/ml (SD=15.49). Results regarding total IgG are consistent with those described in other species with similar type of placentation. HCAbs represented 43% of colostral IgG and IgM concentration presented values that were much higher (two and three times respectively) than those described for species with similar type of placentation. Conclusions: This study is the first which evaluate amounts of each immunoglobulin isotype in colostra of llamas. We described a higher value of IgM in in comparison with species with similar placentation type. The IgG1 isotype, which represents the conventional isotype, is the more abundant antibody in colostra, being the HCAbs the second important fraction. This information may be useful in the management of neonates with failures in the transference of maternal passive immunity by colostra.