Bronchoalveolar lavage hyaluronan leves inhibition by corticosteroid administration in a murine asthma model

ERNST G, LOMPARDÍA S, CORDO RUSSO R, GENTILINI V, VENTURIELLO S, GALÍNDEZ F, GRYNBLAT P, HAJOS SE.

INFLAMMATION RESEARCH

BIRKHAUSER VERLAG AG

Lugar: BASEL; Año: 2012 vol. 61 p. 1309 - 1317

1023-3830

Objective To analyze the effect of corticosteroid administrationon the concentration of hyaluronan (HA) inbronchoalveolar lavage (BAL) in a murine model ofeosinophilic airway inflammation and to study the mechanismsinvolved.Materials and methods Untreated-mice or mice treatedwith 1 lg/g/day betamethasone (Bm) or 0.25 lg/g/day-1budesonide (Bd) were sensitized and challenged withDermatophagoides pteronyssinus (Dp) or saline (controlgroup). The concentration of HA in BAL was determinedby ELISA. In vitro migration assays were performed usinga Boyden chamber and the expression of HA synthases(HAS) was analyzed by RT-PCR.Results We found a significant increase (P.01) in thelevels of HA in BAL from Dp-treated mice that was preventedby Bm or Bd. Corticosteroids also inhibited theincrease in HAS expression, and the phosphorylation ofAkt and ERK in the lungs of challenged mice. Finally, wefound that low molecular weight HA induces the chemotaxisof BAL cells in vitro through a mechanism mediatedby CD44.Conclusion We conclude that corticosteroids prevent theincrease in HA in BAL from Dp-challenged mice. This effect is associated with reduced expression of HAS andreduced phosphorylation of Akt and ERK in the lungs ofchallenged mice.