IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
artículos
Título:
Comment on “A Role for Immature Myeloid Cells in Immune Senescence”
Autor/es:
FERRARI A; CELA EM; GONZÁLEZ MAGLIO DH
Revista:
JOURNAL OF IMMUNOLOGY
Editorial:
AMER ASSOC IMMUNOLOGISTS
Referencias:
Lugar: Bethesda; Año: 2011 vol. 187 p. 6167 - 6167
ISSN:
0022-1767
Resumen:
Letter to the Editor   Sir:                 We have recently read a paper published in the JI entitled ‘A Role for Immature Myeloid Cells in Immune Senescence’ [1], and found an experimental procedure we believe should be discussed. In this paper, as well as many others by the same authors –also in the JI and other renowned journals [2-3]– serum specific antibodies are quantified by ELISA. According to our knowledge this could be a misconception, given that we believe ELISA cannot be used for that purpose. Polyclonal antisera raised against the same antigen could differ both in the affinity and the concentration of the specific antibodies. The main premise of quantification is that sample and reference standard must share all biological and physicochemical properties, being the analyte concentration the only unknown factor. The authors have not declared what they have used as the ‘reference standard’, not in the paper that gives rise to this letter, nor in the others published before. Considering that the samples are polyclonal antisera, we believe there is no way to construct such standard curve that allows expressing the results as a concentration of specific immunoglobulins. Therefore, we think that the only reliable information that can be obtained from this sort of assays is a ‘relative potency’ value, commonly expressed as titer of specific antibodies. We certainly feel this issue does not affect the quality of the work by Enioutina et al., but we do think the quantitative aspects deserve further discussion.   Dr. Alejandro Ferrari Bioch. Eliana Cela Dr. Daniel González Maglio Humoral Immunity Research Institute Pharmacy and Biochemistry School CONICET – University of Buenos Aires   References [1] Enioutina E.Y., Bareyan D., and Daynes R.A. 2011. A Role for Immature Myeloid Cells in Immune Senescence. J. Immunol. 186: 697-707. [2] Enioutina E.Y., Visic D.M., and Daynes R.A. 2002. The induction of systemic and mucosal immunity to protein vaccines delivered through skin sites exposed to UVB. Vaccine 20:2116–2130. [3] Enioutina E.Y., Bareyan D., and Daynes R.A. 2009. TLR-Induced Local Metabolism of Vitamin D3 Plays an Important Role in the Diversification of Adaptive Immune Responses. J. Immunol. 182:4296-4305