IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
artículos
Título:
Comment on A Role for Immature Myeloid Cells in Immune Senescence
Autor/es:
FERRARI A; CELA EM; GONZÁLEZ MAGLIO DH
Revista:
JOURNAL OF IMMUNOLOGY
Editorial:
AMER ASSOC IMMUNOLOGISTS
Referencias:
Lugar: Bethesda; Año: 2011 vol. 187 p. 6167 - 6167
ISSN:
0022-1767
Resumen:
Letter to the Editor
Sir:
We have recently read a paper
published in the JI entitled A Role for Immature Myeloid Cells in Immune
Senescence [1], and found an experimental procedure we believe should be
discussed. In this paper, as well as many others by the same authors also in
the JI and other renowned journals [2-3] serum specific antibodies are
quantified by ELISA. According to our knowledge this could be a misconception, given
that we believe ELISA cannot be used for that purpose.
Polyclonal
antisera raised against the same antigen could differ both in the affinity and
the concentration of the specific antibodies. The main premise of
quantification is that sample and reference standard must share all biological
and physicochemical properties, being the analyte concentration the only
unknown factor. The authors have not declared what they have used as the
reference standard, not in the paper that gives rise to this letter, nor in
the others published before. Considering that the samples are polyclonal
antisera, we believe there is no way to construct such standard curve that
allows expressing the results as a concentration of specific immunoglobulins. Therefore,
we think that the only reliable information that can be obtained from this sort
of assays is a relative potency value, commonly expressed as titer of
specific antibodies.
We
certainly feel this issue does not affect the quality of the work by Enioutina
et al., but we do think the quantitative aspects deserve further discussion.
Dr. Alejandro Ferrari
Bioch. Eliana Cela
Dr.
Daniel González Maglio
Humoral Immunity Research Institute
Pharmacy and Biochemistry School
CONICET University of Buenos Aires
References
[1] Enioutina E.Y., Bareyan D., and Daynes R.A. 2011. A Role for
Immature Myeloid Cells in Immune Senescence. J. Immunol. 186: 697-707.
[2] Enioutina E.Y., Visic D.M., and Daynes R.A. 2002. The induction of
systemic and mucosal immunity to protein vaccines delivered through skin sites
exposed to UVB. Vaccine 20:21162130.
[3] Enioutina E.Y., Bareyan D., and Daynes R.A. 2009. TLR-Induced Local
Metabolism of Vitamin D3 Plays an Important Role in the Diversification of
Adaptive Immune Responses. J. Immunol. 182:4296-4305