IQUIMEFA   05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Fibroblast behaviour within silica nanoparticles-collagen composite hydrogels
Autor/es:
MARTÍN F. DESIMONE; CHRISTOPHE HÉLARY; GERVAISE MOSSER; MARIE-MADELEINE GIRAUD-GUILLE; JACQUES LIVAGE; THIBAUD CORADIN
Lugar:
Tours, Francia
Reunión:
Jornada; Journées Sol-Gel 2010: Sol-Gel, Nanomatériaux et Énergie; 2010
Institución organizadora:
la Société Savante Internationale Sol-Gel
Resumen:
The aim of this work was to develop a new cell based bio-hybrid material based on normal human dermal fibroblast incorporated into silica nanoparticles-collagen composite hydrogels. Cellularized hydrated collagen gels are commonly obtained using the method developed by Bell et al., (1979)1, consisting in the neutralization of diluted acid soluble collagen solutions extemporary prior to cell addition. One of the main limitations in using those types of collagen gels as biomedical materials is related to their strong contraction upon ageing. Recently, we reported a suitable method to generate materials with limited contraction effect upon aging (Desimone et al., 2010)2. It relied on the simultaneous formation of hybrid silica/collagen hydrogels, under conditions compatible with viable cell encapsulation. In the present work, we have use silica nanoparticles of 12 nm and 80 nm to prepare the composites taking advantage of the fact that they do not interfere in the self-assembly process of the collagen molecules during their incorporation. Cell viability and proliferation during 21 days was estimated by MTT-tests. The remodelling activity of the cells was monitored by the gelatine hydrolysis activity of the MMP-2 enzyme. The effect of culture cells in the different composites hydrogels was further analyzed by histological and immunohistological methods, SEM and TEM. The new hybrids present a lower silicate dissolution rate than the former hybrids and, hence, avoid the contraction to a greater extent. Noteworthy, the proliferation of fibroblast cultured was also greater in this new material. These results suggest that these new composites are promising materials for targeted biomedical applications such as biological dressings, where fibroblast proliferation within a biocompatible matrix with controlled biodegradation is necessary to stimulate healing. References: 1E. Bell, B. Ivarsson and C. Merrill, Proc. Natl. Acad. Sci., USA, 1979, 76, 1274. 2M.F. Desimone, C. Hélary, G. Mosser, MM. Giraud-Guille, J. Livage and T. Coradin,  J. Mater. Chem., 2010. DOI: 10.1039/b921572g