Sol-gel chemistry for new material development and capillary electrophoresis applications

MV TUTTOLOMONDO; ME VILLANUEVA; GJ COPELLO; SA GIORGIERI; MF DESIMONE; LE DIAZ

Jurere Beach Village, Florianópolis

Simposio; 16th Latin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical and Industrial applications of capillary electrophoresis and microchip technology; 2010

The sol-gel process has several well-known advantages such as choice of high purity precursors (monomers or condensed species), homogeneity of the obtained material with different shapes (i.e., gels, films, particles) and especially the possibility of making hybrids and composite materials with new chemical and mechanical properties, conductivity and permeability. The sol-gel process involves the addition of a colloidal suspension (sol) of a polymer-forming precursor to a solution of an active material. Further processing of the sol results in the formation of an amorphous glassy like matrix (gel) in which the active material is entrapped. The versatility of sol-gel chemistry  allows producing a wide range of organic-inorganic hybrid materials with numerous promising applications. Moreover, the mild conditions associated with sol-gel chemistry allow the successful immobilization of a broad range of enzymes and living cells. The ability to control the structure of these materials from the nanometric scale and the possibility to incorporate biological systems retaining their functionality or recognition capability will allow developing advanced materials. Herein, we present the surface modification glass slides and glass beads functionalized with APTES, which provide NH2 groups available for reaction with glutaraldehyde or divinylsulphone. Afterwards, protein A was covalent bounded and the system was evaluated for the adsorption of g- fraction from human serum using capillary electrophoresis.