ORAL ADMINISTRATION OF STEVIOSIDE AS A CARDIOPROTECTIVE NUTRACEUTICAL STRATEGY: AN INSIGHT INTO MITOCHONDRIA STATUS AND ITS RELATIONSHIP WITH PROTEIN KINASE B (AKT)

VÉLEZ, DEBORA E.; PEREGO, JULIANA; MOURGLIA, JULIETA; HERMANN R; MESTRE CORDERO, VICTORIA EVANGELINA; HARRIET SOFIA; MARÍA GABRIELA MARINA PRENDES

Ciudad Autónoma de Buenos Aires

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2020; 2020

SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC), SOCIEDAD ARGENTINA DE FISIOLOGÍA (SAFIS), SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI)

Stevioside (S), a diterpenoid glycoside, is the main non-caloricsweetener extracted from Stevia rebaudiana Bertoni leaves. In previousstudies, we demonstrated that the oral administration of S improvedthe recovery of hearts subjected to ischemia-reperfusion (IR),and increased phosphorylation of Akt and GSK3β. These effectswere partially reverted by the administration of wortmannin (W), anupstream inhibitor of Akt.Since mitochondrial dysfunction plays a key role in IR injury, weaimed to investigate the effects of oral administration of S (168mg/kg/15days) on several mitochondrial parameters from Langendorff-perfused rat hearts subjected to I-R.Hearts from female Wistar rats (200-250g) fed ad libitum were used.W (100nM) was added 15 minutes before I. The mitochondrial ultrastructurewas analyzed by electron microscopy, the measurement ofmitochondrial ATP synthesis was performed by the luciferin-luciferasemethod and calcium-triggered mitochondrial swelling was determinedas % of light scattering decrease at 540nm (%LSD). We alsostudied calcium retention capacity (CRC) by exposing mitochondriato small pulses of calcium using the fluorescent dye: Calcium Green-5N. ANOVA, n=8/group.Results showed, at the end of reperfusion, an increase in mitochondrialATP synthesis rate of hearts treated with S (C:66.3±6.5,W:59.5±6.1, S:87.3±3.7*, S+W:64.6±6.9 nmol/min/mg of mitochondrialprotein; *p