IQUIMEFA   05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Liposomal Gramicidin S: a biocompatible nanoformulation against bacteria
Autor/es:
ANTEZANA PABLO E.; MARTINI FLORENCIA; BELLINO MARTIN G.; MUNICOY SOFIA; DESIMONE MARTIN F
Lugar:
New Mexico
Reunión:
Conferencia; #LatinXChem Twitter Conference 2020; 2020
Resumen:
Multidrug resistance of bacteria is nowadays a huge worldwide challenge. Gramicidin S (GraS) is an amphiphilic peptide that has advent as an effective alternative antibiotic due to its distinctive mechanism of action. However, its applicability is restricted due to its killing effect on eukaryotic cells and low aqueous-solubility. In this work, a novel water-soluble peptide formulation with bactericidal activity was developed by the incorporation of Gramicidin S in the lipid bilayer of liposomes (L-GraS). As a result, GraS now included in the lipid vesicles was stabilized in aqueous medium and showed antimicrobial activity against Gram-positive bacteria (Staphylococcus aureus). Significantly, L-GraS revealed an enhanced biocompatibility for mammalian cells in comparison with the free peptide treatment. These results were also analyzed using molecular dynamics simulations that shed light on the collective behavior between GraS peptides and liposomes from a molecular approach. Therefore, the liposomal GraS nano-system we present here is an interesting alternative to conventional antibiotics, expanding its therapeutic horizon as a novel biocompatible antimicrobial nanoformulation.