Efectos inmunomoduladores in vitro de un extracto acuoso de Larrea divaricata cav. en células dendríticas y epiteliales humanas.

R DAVICINO; L TISCORNIA; V SANCHEZ; C ANESINI; BOLLATI-FOGOLIN M

Montevideo, Uruguay

Jornada; Internacional, IV LATIN AMERICAN SEMINAR ON CELL CULTURE TECHNOLOGY (IV SLATCC); 2010

Instituto Pasteur Montevideo

Larrea divaricata (Zygophyllaceae) is a South American  plant  widely  distributed in Argentina. It is used in folk medicine for treatment of  arthritis, inflammation, gastric disturbance, venereal diseases,  tuberculosis and in  healing sores and wounds. It possesses a well  documented immunomodulatory, anti-inflammatory and antitumoral action in  cells derived from rats. Dendritic cells are essential components of  innate immunity that regulate the development of adaptative immune  response and also collaborate in the modulation of T cells responses.  By other way, HT 29-NFκB-hr GFP cells are used to evaluate anti-inflammatory and antitumoral actions of drugs. The aim of this study was to study the immunomodulatory, anti-inflammatory  and antitumoral actions of an aqueous extract of the plant (AE) on  dendritic cells induced from human monocytes of peripheral blood and on  human HT 29-NFκB-hr GFP.  On dendritic cells were studied:  phagocytosis, cytokines production ( IL8, IL6 and IL10),  and HLA-DR, CD86  and HLA-DR/CD86 expression (by flow cytometry) and in HT29 cells,   cytokines (IL-8) ( by flow citometry) , cell viability ( by MTT assay), apoptosis ( by flow cytometry with annexin) and  NFκB translocation  by GFP fluorescein expression. In dendritic cells: AE did not modify  phagocytosis but increased the expression of HLA-DR/C86 and IL8, IL6 and  IL10 production. In HT cells: AE decreased the translocation of NFκB. Also decreased cell viability a and IL8 production induced by TNF   and induced cells to apoptosis. It can be concluded that, AE exerted an   immunodulatory effects on dendritic cells, promoting cell activation by   the production of anti and pro-inflammatory cytokines. Moreover, exerted  antitumoral activity on HT tumor cells as well as demonstrated  anti-inflammatory action. These results confirmed the immunomodulatory  action of Larrea divaricata   on human cells.