IQUIMEFA   05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Mode of action of the sesquiterpene lactone eupatoriopicrin on Trypanosoma cruzi
Autor/es:
PUENTE V; SÜLSEN V; ELSO O; SOSA M; BARRERA P; LOMBARDO E
Lugar:
Mar del Plata
Reunión:
Congreso; Reunión Anual de Sociedades de Biociencias, XXXI Reunión Anual de la Sociedad Argentina de Protozoología (SAP); 2019
Institución organizadora:
Sociedades de Biociencias
Resumen:
Eupatoriopicrin is a sesquiterpene lactone isolated from Stevia maimarensis (Asteraceae), an endemic plant species from northern Argentina. This compound has shown promising trypanocidal activity and selectivity against epimastigotes, trypomastigotes and amastigotes of Trypanosoma cruzi. Taking into account that other sesquiterpene lactones isolated by our group exerted its tripanocidal activity by sterol biosynthesis inhibition, interacting with hemin and/or producing mitochondrial dysfunction, the objective of this work was to assess the effect of eupatoriopicrin on these T. cruzi targets. The interaction of this sesquiterpene lactone with hemin was examined under reducing and non- reducing conditions by measuring modifications in the hemin Soret band absorption. The mitochondrial function was assessed by means of rhodamine 123 staining and by measurement of NADH-cytochrome c reductase and succinate dehydrogenase activities. Lipids profile was analyzed by TLC and ultrastructural changes were determined by transmission electron microscopy.Eupatoriopicrin did not interact with hemin, but affect the functionality of the mitochondria and the lipids profile in the parasite. TLC analysis showed that eupatoriopicrin did not affect sterol biosynthesis but led to triglyceride accumulation. Microscopy images analysis showed the presence of lipid droplets in parasite cytoplasm. Based on our results, eupatoriopicrin would exert its activity by producing mitochondrial dysfunction and by altering the lipid profile in T. cruzi