LIPOSOME LOADED COLLAGEN BASED BIOMATERIALS WITH ANTIMICROBIAL ACTIVITY

MUNICOY SOFIA; ANTEZANA PABLO E.; DESIMONE MARTIN F; BELLINO MARTIN G.

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencia; 2019

SAIC, SAI, SAFIS

Hydrogels are promising materials in the field of biomedicine due to its biocompatibility and biodegradability. Liposomes (L) have proved to be an effective vehicle as they are not toxic, biodegradable and can encapsulate and control release of different drugs. Silver nanoparticles (AgNPs) have been demonstrated to have antibacterial activity. In order to overcome the challenge of developing a long-term antimicrobial material, we report a non-conventional biomaterial with prolonged bactericidal effect based on the incorporation of liposomes encapsulating silver nanoparticles in collagen hydrogels. AgNPs were synthesized by a reduction method and then incorporated in liposomes (L-AgNPs) by the lipid film hydration and extrusion technique. Collagen hydrogels were prepared by exposing a collagen solution extracted from rat tails to a saturated atmosphere of ammonia to induce gelation. Antimicrobial collagen-based scaffolds were prepared by adding AgNPs-containing liposomes suspensions to the collagen gels (Col-L-AgNPs). The optical properties of AgNPs were monitored by UV?vis spectroscopy. The morphology of AgNPs and L-AgNPs was studied by TEM and the structure of collagen gels before and after incorporation of L-AgNPs was analyzed by SEM. The antibacterial efficiency of Col-L-AgNPs was then evaluated on Gram-positive (S. aureus) and Gram-negative (P. aeruginosa) bacteria and cytotoxicity towards mammalian cells was studied.As a result, AgNPs exhibited well dispersed spherical morphology with an effective diameter between 5 and 20 nm. The L-AgNPs showed an effective diameter of approximately 410 nm. Col-L-AgNP showed an important bactericidal activity against both bacteria and did not affect cell viability. Based on these results, Col-L-AgNPs is promising as a new material that conserves a strong bactericidal activity and biocompatible properties for 72 h which is especially attractive for wound dressing as it does not need to be replaced repetitively.