Antioxidant action of Cannabis sativa resin and CBD and synergism induced by Larrea divaricata and Tilia x viridis extracts

FRESIA MELINA SILVA SOFRÁS; MARÍA ROSARIO ALONSO; IGNACIO PERALTA; CATALINA VAN BAREN; MALÉN SAINT MARTIN; LAURA COGOI; CLAUDIA ANESINI

Mar del Plata

Congreso; Reunion conjunta de sociedades de Biociencia, XLXI Reunion Anual de la Sociedad de Farmacología Experimental (SAFE); 2019

Sociedad argentina de Farmacología Experimental

Cannabis sativa L. (Cannabaceae) is a medicinal plant used as anticonvulsant in refractory epilepsy in kids. Convulsions induce oxidative stress (OS) in cerebral tissues which affects the normal function of the central nervous system altering cognitive functioning. The synergistic effects of plants could not only improve therapeutic effects but also mitigate adverse effects. Larrea divaricata Cav.(Zygophylliaceae) is an autochthonous plant with a well documented antioxidant activity and Tilia x viridis ssp moltkei (Tiliaceae) is a plant used for its anxiolytic effects. The purpose of this work was to evaluate the antioxidant activity of an ethanolic extract (EEC) of C. sativa, studying the participation of the cannabinoid CBD in its action and to evaluate for the first time the possible synergistic combination of EEC with aqueous extracts of L. divaricata (AEL) and Tilia x viridis (AET).The antioxidant activity was determined by the capacity of scavenging the radical 1,1-diphenyl-2-picryhydrazyl (DPPH) by a spectrophotometric assay. CBD was identified and quantified by HPLC.Quantification of CBD: 0.273 mg CBD/mg EEC. All the extracts presented antioxidant activity. Results expressed as inhibitory medium concentration IC50 (µg/ml) represent Mean ± SEM of three experiments made by duplicate (*P< 0.05; **P