IQUIMEFA   05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
CARDIOPROTECTION CARRIED OUT BY ORAL ADMINISTRATION OF STEVIOSIDE; AN INSIGHT INTO MITOCHONDRIA STATUS AND ITS RELATIONSHIP WITH PROTEIN KINASE B (AKT).
Autor/es:
VELEZ, D.; HARRIET, S.; PEREGO, J.; HERMANN, R.; MESTRE CORDERO, V.; MOURGLIA, J.; MARINA PRENDES, MG.
Lugar:
Mar del Plata
Reunión:
Congreso; Reunión Anual de Sociedades Biocientíficas; 2019
Institución organizadora:
Sociedad Argentina de Investigación Clínica, la Sociedad de Farmacología Experimental, la Sociedad Argentina de Biología, la Sociedad Argentina de Protozoología, la Asociación Argentina de Nanomedicinas, La Asociación Argentina de Ciencia y Tecnología de
Resumen:
In previous studies carried out in our laboratory, we demonstrated that oral administration of stevioside (S), a mayor component of Stevia rebaudiana Bertoni, improved the recovery of contractile activity in hearts subjected to ischemia-reperfusion (I-R), decreased the infarct size andincreased Akt and GSK3ß phosphorylation. These effects were reverted, at least in part, by the administration of Wortmannin (W), an upstream inhibitor of Akt. Since mitochondrial dysfunction is essential for the pathogenesis of I-R injury, we aimed to investigate the effects of oral administration of S (168 mg/kg for 15 days) in several mitochondrial parameters from Langendorff perfused rat hearts subjected to I-R. Hearts from female Wistar rats (200 ? 250 g) fed ad libitum were used. W(100 nM) was added 15 min before I. Mitochondrial ultrastructure was analyzed by electron microscopy, the measurement of mitochondrial ATP synthesis was made by the luciferin-luciferase method and calcium-triggered mitochondrial swelling was determined as % of light scattering decrease at 540 nm (% LSD). We also studied the calcium retention capacity (CRC) exposing mitochondria to calcium pulses using the fluorescent dye: Calcium Green-5N. ANOVA, n= 8/group. At the end of reperfusion, results showed an increase in mitochondrial ATP synthesis rate of hearts treated with S that was partly canceled by the administration of W (C:66.3 ± 6.5, W: 59.5 ±6.1, S: 87.3 ± 3.7*, S+W: 64.6 ± 6.9 nmol/min/mg mitochondrial protein; *p