Renal oxidative stress in hypertensive rats. Atrial natriuretic peptide chronic treatment.

SAVIGNANO, LUCIA; MOLINARI, AGUSTINA; BOUCHET, GONZALO; AGUIRRE, SOFIA; CANIFFI, CAROLINA; ROMERO, MARIANA; TOMAT ANALIA; ELESGARAY, ROSANA; ARRANZ, CRISTINA; COSTA, ANGELES

Facultad de Farmacia y Bioquímica. UBA

Workshop; International Workshop in oxidative stress and antioxidants; 2009

Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires

&amp;amp;amp;amp;amp;amp;lt;!-- /* Font Definitions */ @font-face {font-family:TTE1BFFBC0t00; panose-1:0 0 0 0 0 0 0 0 0 0; mso-font-charset:0; mso-generic-font-family:auto; mso-font-format:other; mso-font-pitch:auto; mso-font-signature:3 0 0 0 1 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} @page Section1 {size:595.3pt 841.9pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:35.4pt; mso-footer-margin:35.4pt; mso-paper-source:0;} div.Section1 {page:Section1;} --&amp;amp;amp;amp;amp;amp;gt; RENAL OXIDATIVE STRESS IN HYPERTENSIVE RATS.  ATRIAL NATRIURETIC PEPTIDE CHRONIC TREATMENT. Savignano, L; Molinari, A; Bouchet, G; Aguirre, S.; Caniffi, C; Romero, M; Tomat, A; Elesgaray, R; Arranz, C; Costa, M.Á. Cátedra de Fisiología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires. IQUIMEFA-CONICET. mcosta@ffyb.uba.ar The aim of the study was to investigate the effects of chronic infusion with atrial natriuretic peptide (ANP) on renal oxidative stress in spontaneously hypertensive rats (SHR). Methods: 8 weeks-old SHR and Wistar Kyoto (WKY normotensive rats) were infused (14 days, subcutaneous osmotic pumps) with ANP (100 ng/hr/rat) or saline (S). After experimental period, we determined in kidney: thiobarbituric acid-reactive substances (TBARS, nmol/mg protein), glutathione concentration (mg/mg protein), and activity of: glutathione peroxidase (GPx, pmol/min.mg protein), catalase (CAT, pmol/mg protein) and superoxide dismutase (SOD, USOD/mg protein). Results: TBARS Glutathione GPx CAT SOD WKY S 0,113±0,015 0,271±0,038 1,449±0,230 0,847±0,102 7,691±0,505 WKY ANP 0,098±0,010 0,398±0,028* 1,890±0,302 0,956±0,098 8,021±1,654 SHR S 0,162±0,025* 0,178±0,046* 3,315±0,225* 0,951±0,105 7,098±0,653 SHR ANP 0,082±0,009# 0,374±0,027# 3,920±0,845 1,101±0,142 8,452±0,779 *p< 0,01 vs WKY S; #p<0,01 vs SHR S. SHR shown higher levels of TBARS and GPx activity than WKY rats. Renal glutathione concentration was lower in hypertensive rats than in normotensive ones. CAT and SOD basal activities were similar in both groups. In WKY rats, ANP treatment increased glutathione concentration. In hypertensive rats, ANP chronic infusion diminished TBARS and increased glutathione renal concentration, indicating a reduction in renal oxidative stress. Conclusion: Chronic treatment with ANP improved, almost in part, renal oxidative damage in this model of hypertension in rat.