CELL ADHESION AND BIOCOMPATIBILITY OF PVA SPONGES CROSSLINKED WITH HEXAMETHYLENE DIISOCIANTE FOR REGENERATION OF BONE TISSUE.

OLIVETTI, CHRISTIAN EZEQUIEL; ALVAREZ, GISELA SOLANGE; ALVAREZ ECHAZÚ MARÍA INÉS; DESIMONE MARTÍN FEDERICO; PERNA ORIANA; GARCIA CUNIGLIO SABRINA

Buenos Aires

Jornada; XX JORNADAS ANUALES DE LA SOCIEDAD ARGENTINA DE BIOLOGÍA (SAB), XVII JORNADAS DE LA SOCIEDAD URUGUAYA DE BIOCIENCIAS (SUB)-Segundas Jornadas Rioplatenses de Biología.; 2018

PVA sponges crosslinked with hexamethylene diisocyanate (HDI) were developed to obtain hydrophilic and slowly-degradable scaffolds for biomedical purposes with good biocompatibility and better mechanical properties. The obtainedPVA-crosslinked with different concentrations of HDI was characterized by IR spectroscopy where it was possible toobserve a reduction in the peaks intensity at 3300 cm -1 corresponding to O?H stretching, indicating the effective reactionof HDI with PVA hydroxyl groups. It was also possible to determine that the NCO stretching peak at 2280 -1 disappearedafter the reaction with PVA except for the highest concentration. Also we observed an increment in the signal at 1750 cm -1corresponding to free C=O stretching as the mobility of molecular chains was restrained by the reaction between ?OH and?NCO, which prevents the formation of hydrogen bonds. The materials were also observed by electron microscopy andthe increment in nitrogen content due to HDI was measured by EDX. The swelling radio and degradation was evaluatedwith the intention to demonstrate the long-term physical integrity of the material. PVA sponges swelling ratio was 400%while for HDI crosslinked sponges this value was modified to 150%. Materials with the highest percentage of HDI did notshow significant degradation after three weeks of incubation. Furthermore, an osteoblastic cell line 3T3 E1 was used toevaluate cell adhesion to the materials and their cytocompatibility. According to these studies, PVA is an attractivematerial for these applications because of its biocompatibility and low protein adsorption properties which result in lowcell adhesion compared with other hydrogels. However, this fact may complicate its application in regenerative medicineas cell scaffold for osteoblast colonization. For this reason, PVA- crosslinked hydrogels were incubated with collagen Iand IV to increase cell adhesion to the materials. It was possible to observe that the system was not toxic for the cells anda significant increment between 4 and 5 times with collagen IV coating extracted from placenta was obtained but not suchresults were obtained for collagen type I.