Isolation and characterization of compounds present in the extract of Eupatorium hecathantum (D.C) Bak. (Asteraceae) with trypanocidal activity

BIVONA A; GROSO C; MALCHIODI E; ELSO O; SANCHEZ ALBERTI A; CERNY N; CLAVIN M; BORGO J; SELENER M; SÜLSEN V

CABA

Congreso; Drug Discovery for Neglected Diseases International Congress 2018; 2018

Instituto de Quimica y Metabolismo del Farmaco (IQUIMEFA) (UBA-CONICET)

Eupatorium hecathantum (D.C) Bak. is a native medicinal species that grows in Bolivia, South Brasil and North up to Center of Argentina. Pilaga originary communities from Argentina use this plant as antitussive and for gastrointestinal pain [1]. Toba communities employ it for tooth pain, diarrhea [2], for parasitism treatment and skin disorders like gangrene and ulceration [3]. Dichloromethane extracts of E. hecathanthum and other medicinal species of the genus Eupatorium were screened for antiprotozoal activity against Trypanosoma cruzi epimastigotes. E. hecathanthum dichlorometane extract was one of the most active one against T. cruzi epimastigotes. It produced an 85% inhibition of the growth of the parasites at the extract concentration of 100 mg/ml. Growth inhibition of parasites was assayed using the (3H) thymidine uptake. Parasites were adjusted to a cell density of 1.5 x 106/ml and cultured in the presence of 100 and 10 mg/ml of extracts 72 h. Benznidazole (5 to 20 mM, Roche) was used as positive control. The percentage of inhibition was calculated as 100 - {[(cpm of treated parasites)/(cpm of untreated parasites)] x 100}.HPLC-DAD and TLC analysis showed the presence of methoxilated flavones, flavonols and terpenoids. Among them, the flavones jaceosidin and nepetin with reported trypanocidal effect [4], were identified. Isolation and identification of other flavonoids and terpenoids is being carried out, in order to find the compounds responsible of the trypanocidal activity. References 1.Filipov A. (1994). Medicinal Plants of the Central Chaco. Journal of Ethnopharmacology 44: 181-193.2.Vuoto P. M. (1981). Plantas utiles entre los Toba- TakseK. Entregas del Instituto Tilcara 10:12-76. 3.Martinez G., Barboza G. (2010). Natural pharmacopoeia used in traditional Toba medicine for the treatment of parasitosis and skin disorders (Central Chaco, Argentina). J. of Ethnopharm. 132: 86-100.4.Clavin M. (2012). Propiedades biológicas de especies medicinales argentinas de Eupatorium. Aislamiento de principios bioactivos de Eupatorium arnottianum?. Tesis Doctoral. Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica.