Study of cardiac function and renin-angiotensin system in a fetal programming model of moderate zinc deficiency in adult rats

ALADRO MANUELA; GONANO LUIS; ELESGARAY ROSANA; TOMAT ANALÍA; GOBETTO, MARÍA NATALIA; MENDES GARRIDO ABREGÚ, FACUNDO; GIRONACCI MARIELA; CANIFFI CAROLINA; JURIOL, LORENA VANESA; MORELL MALENA; VILA PETROFF MARTÍN; ARRANZ CRISTINA

Río de Janeiro

Congreso; IUPS 38th World Congress; 2017

International Union of Physiological Sciences and Sociedad Brasileira de Fisiologia

Moderate zinc deficiency during intrauterine and postnatal growth induces low birth weight, hypertension and cardiovascular dysfunction in adult male rats. The cardiac renin angiotensin system (RAS) exerts an important modulating effect on cardiac function and development.This study?s aim was to evaluate cytosolic Ca2+ management and cell shortening in isolated cardiomyocytes; as well as the RAS in the left ventricle (LV) of male and female adult rats exposed to a low zinc diet during fetal life, lactation and/or growth.Female Wistar rats received low (L:8ppm) or control (C:30ppm) zinc diet during pregnancy up to weaning. After weaning, male (m) or female (f) L offspring, received low (l) or control (c) zinc diet during 60 days (Llm, Llf, Lcm, Lcf). C offspring continued with control (c) zinc diet (Ccm, Ccf). At day 81 of life we evaluated in LV: transient increase of cytosolic Ca2+ during systole (TICa: Fura-2 (340/380nm)) and shortening in electrically stimulated isolated cardiomyocytes (CS:% of max length, Epifluorescence microscopy), ACE mRNA expression (ACE/GADPH, RTqPCR), expression of AT1 and AT2 receptors (optical density/actin WB) and concentrations of Angiotensin II and 1-7 (AII, A1-7 nM/g tissue, RIA). Values are mean±SEM, n=6/group. Two-way ANOVA, Bonferroni post-test. *p