Potential actives from native argentine and south american medicinal plants in phytocosmetics

NADINIC, JELENA; ULLOA, JERÓNIMO; DEBENEDETTI, SILVIA; LOPEZ VARGAS, PAOLA; MOSCATELLI, VALERIA; MEC CAM, LAURA; REDKO, FLAVIA; MUSCHIETTI, LILIANA; ISLA MARIA INES; ZAMPINI, CATIANA; D´BENEDETTO, FABRINA; BUGGE, SILVINA; PERALTA, IGNACIO; RETTA, DAIANA; VAN BAREN, CATALINA; GONZALEZ, SILVIA; AGNESE, NADIA; DEKKER, MARIANA; GASTALDI, BRUNO

Patras

Congreso; - 5TH International & Phytotherapy Congress; 2017

Potential actives from native argentine and southamerican medicinalplantsin phytocosmeticsJelenaNadinic, Daiana Retta, Liliana Muschietti, Jerónimo Ulloa, Catalina Van Baren,María Inés Isla, Silvia Debenedetti, Silvia B. González, Catiana Zampini, PaolaLópez Vargas, Nadia Agnese, Fabrina D´Benedetto, Valeria Moscatelli, MarianaDekker, Silvina Bugge, Laura MecCam, Bruno Gastaldi, Ignacio Peralta, FlaviaRedko Background: We have been working in a research project entitled"Traditional Medicinal Plants as Source for Phytocosmetic andDermatological Products" whose main objective is to produce standardizedextracts with proven biological properties and to evaluate their end productsunder clinical studies. For this purpose, we performed a survey of native plantsof Argentina and South America used in the traditional medicine for thetreatment of skin ailments, bruises and inflammation. Based on differentcriteria, we selected thirteen different species: Passiflora caerulea (Pc), Gentianellaachalensis (Gach), Gleditsia amorphoides (Gam), Achyrocline satureioides (As), Zuccagniapunctata (Zp), Baccharis spicata (Bs), Tipuana tipu (Tt), Chorisia speciosa (ChS), Erythrinacrista-galli (Ecg), Buddleja globosa (Bgl), Solidago chilensis (Sch), Smallanthussonchifolius (Ss) and Punica granatum (Pg). These species were previouslystudied on their phytochemical composition and their toxicological andbiological activities. Results: The TLC analysis of ethanolic 6 extract (E80)Tt-E80, Chs-E80, Ecg-E80, Bgl-E80, Sch-EE80, Pg-EE80 and propilen glycolicextracts (PPG): Pc-PPG, Gach-PPG, were performed in CS1, CS2 and CS3; while Zp-E80,Zp-PPG, Bs-E80 and Gam-E80 were performed using CS3. As-E80 was analyzed in CS4,whereas Gach-PPG was also analyzed using CS4 and CS5. SS-E80 was tested using CS4and CS6. Through TLC analysis, phenolic compounds, mainly flavonoids were determinedas the main components of the Pc-PPG, Gach-PPG, Chs-E80, Ecg-E80, Bgl-E80, Sch-E80,Pg-E80 and SS-E80. On the other hand, the TLC analysis of Zp-E80, Bs-E80, Gach-PPGand Gam-E80 allowed the detection of terpenes. The average total phenolic contentdetermined by the Folin Ciocalteu assay of the ethanolic extracts was: T. tipuana 0.19 g/100ml, C. speciosa 0.03 g/100ml, B. spicata 0.28 g/100ml, B. globosa 0.53 g/100ml, E. crista-galli 0.03 g/100ml and S. chilensis 0.22 g/100ml. The DPPH? radical scavenging activity(%) was investigated in the ethanolic extracts (80%) at two concentrations (0.1and 1 mg/ml). The highest activity (at 0.1 and 1 mg/ml, respectively) wasobserved with: G. amorphoides: n.d. and 79.91%, Z. punctata: 79.67% and 74.95%, B. spicata: 81.48% and 77.98%, T. tipu: 67.90% and 69.31%, B. globosa: 76.07% and 71.66%; and for P. granatum: 12.05% and 79.84%, respectively. The development ofthe cosmetic formulations is performed by postgraduate students fromUniversidad de Belgrano, Universidad de Buenos Aires and Asociación Argentinade Químicos Cosméticos (AAQC), an associated collaborator in the project.