IQUIMEFA   05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
COMPARATIVE STUDY OF THE ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF TWO SPECIES OF Urera aurantiaca FROM DIFFERENT GEOGRAPHICAL AREAS: THEIR RELATION TO POLYPHENOLS CONTENT
Autor/es:
IGNACIO PERALTA; CARLA MARRASSINI; CLAUDIA ANESINI
Lugar:
Buenos Aires
Reunión:
Congreso; Reunion conjunta de sociedades de Biociencia, XLIX Reunion Anual de la Sociedad de Farmacología Experimental (SAFE); 2017
Institución organizadora:
Sociedad Argentina de Farmacología Experimental
Resumen:
COMPARATIVE STUDY OF THE ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF TWO SPECIES OF Urera aurantiaca FROM DIFFERENT GEOGRAPHICAL AREAS: THEIR RELATION TO POLYPHENOLS CONTENTUrera auratiaca is an Argentinean species that has been traditionally used to treat symptoms of inflammation. The aim of this study was to determine comparatively the antioxidant and anti-inflammatory effects of two species of Urera aurantiaca coming from different geographical areas of Argentina: Salta (S) and Misiones (M). Methanol extracts were prepared to obtain polyphenolic compounds. Both extracts showed antioxidant activities and in vitro anti-inflammatory activities, results represent mean ± ESM of three experiments made in triplicate: DPPH radical scavenging activity S EC50: 245 ± 40 μg/ml, M EC50: 977 ± 20 μg/ml; Reducing power S EC50: 126 ± 3 μg/ml , M EC50: > 1000 ± 50 μg/ml; Inhibition of lipid peroxidation S EC50: 1862 ± 10 μg/ml, M EC50: 2000 ± 20 μg/ml; SOD like activity S EC50: 537 ± 37 μg/ml, M EC50: 363 ± 24 μg/ml; Inhibition of albumin denaturation S EC50: 812 ± 59 μg/ml, M EC50: > 1000 ± 25 μg/ml; Proteinase inhibitory action S EC50: 10 ± 2 μg/ml, M EC50: 42 ± 4 μg/ml. They also were able to reduce the augmented NO release in activated macrophages but S extract showed the largest inhibition: 76.6% at 500 μg/ml and was able to restore LPS to the basal levels. S extract showed the best antioxidant and anti-inflammatory properties in almost all the tested experiments probably due to the highest polyphenols content (S: 30.9 ± 1.8; M: 14.1± 2.6 GAE/g) and specifically due to the highest flavonoids content (S: 2.10 ± 0.02%; M: 1.49 ± 0.02%). These differences between the extracts are probably related to ambient diverse conditions: temperature, rain, soil composition and ecosystem.