IQUIMEFA   05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Relationship between cardioprotective effects of rosuvastatin (R) and inactivation of gsk3b in rat hearts subjected to ischemia-reperfusion.
Autor/es:
M.G MARINA PRENDES; JULIANA PEREGO; M.G MARINA PRENDES; JULIANA PEREGO; HERMANN, ROMINA; D VELEZ; HERMANN, ROMINA; D VELEZ; M PENIDA; MESTRE CORDERO, VICTORIA EVANGELINA; M PENIDA; MESTRE CORDERO, VICTORIA EVANGELINA
Reunión:
Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017
Resumen:
Previously in our laboratory we could establish that the administration of rosuvastatin in hearts subjected to ischemia-reperfusion (I-RP) generated direct cardioprotective effects. These results were accompanied by increased activation of Akt, attenuation of the opening of the mitochondrial permeability transition pore (MPTP) and preservation of mitochondrial structure. The goal of the present work was to determine the relationship between these events, phosphorylation of GSK3 and the metabolic environment in Langendorff perfused rat hearts subject to IR. Wistar female rats (250-300g body weight) feeding ad libitum were used. Rosuvastatin (R) (3uM) and wortmannin (W), Akt inhibitor (100nM), were added 10 and 15 min before global I respectively. The hearts were subjected to 25 min of I and 60 min of RP. Tissue samples were taken at the end of stabilization and ischemia to determinate lactic acid and glycogen content. The activation profile of GSK3 was studied by western blot. ANOVA, n=8.Lactic acid content were meaningfully lower in those hearts treated with R (µmoles/g dw): C (control): 146.28±6.60*; R: 56.68±6.77; W: 65.31±5.22; R+W: 61.35±4.10 (*p