“IN VITRO” COMPARATIVE EFFECT OF AN AQUEOUS EXTRACT OF LARREA DIVARICATA CAV. AND NDGA ON H2O2 METABOLISM OF NORMAL RATS SUBMANDIBULARY GLANDS

SEBASTIAN TURNER; GABRIELA ZETTLER; ROBERTO DAVICINO; CLAUDIA ANESINI

Rosario, Santa Fé, Argentina

Congreso; XLI reunion anual de la sociedad Argentina de Farmacologia Experimental; 2009

Sociedad Argentina de Farmacología Experimental

L. divaricata possess anti-inflammatory and antioxidant actions. It was studied the effect of L. divaricata aqueous extract (LAE)(1-1000 µg/ml) and NDGA (0.084-3.41µg/ml, concentrations present in the extract) on hydrogen peroxide (H2O2) level and on superoxide dismutase (SOD), catalase (CAT), and  peroxidase (Px), in submandibulary gland of normal Sprague Doyle rats. Results were expressed as X ± SEM of eight determinations performed by duplicate: H2O2 (mM/ g gland), control (C) (4.56 ± 0.4); LAE 500 µg/ml (2.5 ± 0.2 *). Px (U/ml/g gland) C: 0.0086 ± 0.0008,  LAE: 10 µg/ml: 0.0087 ± 0.0008; 100 µg/ml: 0.0088 ± 0.0009; 500 µg/ml: 0.026 ± 0.0010**; 1000 µg/ml: 0.080 ± 0.0080**; CAT (U/ml/g gland) C: 25.82 ± 2; LAE 10 µg/ml: 27 ±  2; 100 µg/ml: 18.74 ± 1*; 500 µg/ml: 10.96 ± 0.8*; 1000 µg/ml: 13 ± 1**. SOD (U/ml/g gland), C: 6.57 ± 0.6; LAE 10 µg/ml: 5.87 ± 0.5; 100 µg/ml: 6.57 ± 0.6; 500 µg/ml: 4 ± 0.38**; 1000 µg/ml: 2.80 ± 0,28**. NDGA:  Px:  0.084 µg/ml: 0.0050 ± 0.0005*; 0.34 µg/ml: 0.011 ± 0.001; 3.41 µg/ml: 0.019 ± 0.0019**. CAT: 0.084 µg/ml: 27.97 ±2; 0.34 µg/ml: 19.2 ± 2*; 3.41 µg/ml: 28.8 ± 2. SOD 0.084 µg/ml: 6.4 ±0.7; 0.34 µg/ml: 0.17 ± 0.01**; 3.41 µg/ml: 0.15 ± 0.01. LAE decreased H2O2 level through an increase of Px activity and a decrease of SOD activity both effect were related to the presence of NDGA and others compounds