Potential actives from native argentine and south american medicinal plants in phytocosmetics

LILIANA MUSCHIETTI ; MARÍA INÉS ISLA; CATIANA ZAMPINI; FABRINA D'BENEDETTO; SILVINA BUGGE; IGNACIO PERALTA; DAIANA RETTA; CATALINA M. VAN BAREN; SILVIA B. GONZÁLEZ; NADIA AGNESE; MARIANA DEKKER; BRUNO GASTALDI; JELENA NADINIC; JERÓNIMO ULLOA; SILVIA DEBENEDETTI; PAOLA LÓPEZ VARGAS; VALERIA MOSCATELLI; LAURA MECCAM; FLAVIA REDKO

Patras, Rio,

Congreso; 5 th International Phytocosmetics and Phytotherapy Congress; 2017

We have been working in a research project entitled "Traditional Medicinal Plants as Source for Phytocosmetic and Dermatological Products" whose main objective is to produce standardized extracts with proven biological properties and to evaluate their end products under clinical studies. For this purpose, we performed a survey of native plants of Argentina and South America used in the traditional medicine for the treatment of skin ailments, bruises and inflammation. Based on different criteria, we selected thirteen different species: Passiflora caerulea (Pc), Gentianella achalensis (Gach), Gleditsia amorphoides (Gam), Achyrocline satureioides (As), Zuccagnia punctata (Zp), Baccharis spicata (Bs), Tipuana tipu (Tt), Chorisia speciosa (ChS), Erythrina crista-galli (Ecg), Buddleja globosa (Bgl), Solidago chilensis (Sch), Smallanthus sonchifolius (Ss) and Punica granatum (Pg). These species were previously studied on their phytochemical composition and their toxicological and biological activities. Results: The TLC analysis of ethanolic 6 extract (E80) Tt-E80, Chs-E80, Ecg-E80, Bgl-E80, Sch-EE80, Pg-EE80 and propilen glycolic extracts (PPG): Pc-PPG, Gach-PPG, were performed in CS1, CS2 and CS3; while Zp-E80, Zp-PPG, Bs-E80 and Gam-E80 were performed using CS3. As-E80 was analyzed in CS4, whereas Gach-PPG was also analyzed using CS4 and CS5. SS-E80 was tested using CS4 and CS6. Through TLC analysis, phenolic compounds, mainly flavonoids were determined as the main components of the Pc-PPG, Gach-PPG, Chs-E80, Ecg-E80, Bgl-E80, Sch-E80, Pg-E80 and SS-E80. On the other hand, the TLC analysis of Zp-E80, Bs-E80, Gach-PPG and Gam-E80 allowed the detection of terpenes. The average total phenolic content determined by the Folin Ciocalteu assay of the ethanolic extracts was: T. tipuana 0.19 g/100ml, C. speciosa 0.03 g/100ml, B. spicata 0.28 g/100ml, B. globosa 0.53 g/100ml, E. crista-galli 0.03 g/100ml and S. chilensis 0.22 g/100ml. The DPPH? radical scavenging activity (%) was investigated in the ethanolic extracts (80%) at two concentrations (0.1 and 1 mg/ml). The highest activity (at 0.1 and 1 mg/ml, respectively) was observed with: G. amorphoides: n.d. and 79.91%, Z. punctata: 79.67% and 74.95%, B. spicata: 81.48% and 77.98%, T. tipu: 67.90% and 69.31%, B. globosa: 76.07% and 71.66%; and for P. granatum: 12.05% and 79.84%, respectively.