Dodecenly succinic anhydride-collagen modified hydrogels

OLIVETTI CHRISTIAN; ALVAREZ GISELA; PERNA ORIANA; ALVAREZ ECHAZU, MI; DESIMONE MARTÍN

Córdoba

Congreso; SAIB 2016; 2016

Dodecenly succinic anhydride (DDSA) was used to modify collagen hydrogels in order to obtain more hydrophobic surfaces. The base catalyzed reaction was performed under ammonia vapor with DDSA solutions ranging from 3 to 25 g/100 ml in ethanol and hydrogels with a concentration of 5 mg/ml of collagen. Different techniques were used to confirm the modifications. In the IR spectra the band at 2884 cm−1 represents the characteristic vibration due to C−H stretching which increases in DDSA modified gels as well as the band at 1662 cm-1 corresponding to the C=O stretching vibration of a carbonyl group. When DSC analysis was performed over hydrated gels, the denaturation temperature obtained was similar for both modified and pure collagen gels. SEM images demonstrate that DDSA esterification does not significantly alter the morphology of pre-formed collagen gels. However, when DDSA was included in the polymerization process of collagen, a remarkable difference was observed.The DDSA-modified gels were used for cell culture with a pre-osteoblastic cell line MC3T3-E1. No toxicity was observed for the material as the number of total cells per well was not significantly different to unmodified collagen gels, showing their biocompatibility. However, a lower cellular adhesion to the surfaces was found as DDSA concentration increased due to the surface hydrophobicity modification.